EU/US Properties Organoleptics Cosmetics Suppliers Safety Safety in use Safety references References Other Blenders Uses Occurrence Synonyms Articles Notes

guanidine HCl
guanidine, hydrochloride (1:1)

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CAS Number: 50-01-1Picture of molecule3D/inchi
Other(deleted CASRN):106946-18-3
ECHA EINECS - REACH Pre-Reg:200-002-3
Beilstein Number:3591990
Molecular Weight:95.53182000
Formula:C H6 Cl N3
BioActivity Summary:listing
NMR Predictor:Predict (works with chrome or firefox)
Category:buffering agents
US / EU / FDA / JECFA / FEMA / FLAVIS / Scholar / Patent Information:
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Physical Properties:
Assay: 95.00 to 100.00
Food Chemicals Codex Listed: No
Melting Point: 182.30 °C. @ 760.00 mm Hg
Flash Point: 32.00 °F. TCC ( 0.00 °C. ) (est)
Soluble in:
 water, 1e+006 mg/L @ 25 °C (est)
Organoleptic Properties:
Odor and/or flavor descriptions from others (if found).
Cosmetic Information:
CosIng:cosmetic data
Cosmetic Uses: buffering agents
Allan Chemical
Guanidine HCl
BOC Sciences
For experimental / research use only.
Guanidine Hydrochloride >98%
Odor: characteristic
Use: Guanidine HCl, the crystalline compound of strong alkalinity formed by the oxidation of guanine, is a normal product of protein metabolism and a protein denaturant.
EMD Millipore
For experimental / research use only.
Guanidine Hydrochloride
Glentham Life Sciences
Guanidine hydrochloride, 99%
Santa Cruz Biotechnology
For experimental / research use only.
Guanidine Hydrochloride ≥99%
Sigma-Aldrich: Sigma
For experimental / research use only.
Guanidine hydrochloride organic base and chaeotropic agent, ≥99% (titration)
Safety Information:
Preferred SDS: View
Hazards identification
Classification of the substance or mixture
GHS Classification in accordance with 29 CFR 1910 (OSHA HCS)
None found.
GHS Label elements, including precautionary statements
Hazard statement(s)
None found.
Precautionary statement(s)
None found.
Oral/Parenteral Toxicity:
intraarterial-guinea pig LDLo 500 mg/kg
Naunyn-Schmiedeberg's Archiv fuer Experimentelle Pathologie und Pharmakologie. Vol. 131, Pg. 171, 1928.

intravenous-guinea pig LDLo 500 mg/kg
Naunyn-Schmiedeberg's Archiv fuer Experimentelle Pathologie und Pharmakologie. Vol. 131, Pg. 171, 1928.

oral-mammal (species unspecified) LDLo 300 mg/kg
Journal of Industrial Hygiene. Vol. 13, Pg. 87, 1931.

intraperitoneal-mouse LD50 500 mg/kg
National Technical Information Service. Vol. AD277-689

oral-mouse LD50 571 mg/kg
Acute Toxicity Data. Journal of the American College of Toxicology, Part B. Vol. 12, Pg. 598, 1993.

oral-rat LD50 475 mg/kg
National Technical Information Service. Vol. AD-A165-747

Dermal Toxicity:
subcutaneous-dog LDLo 200 mg/kg
"Abdernalden's Handbuch der Biologischen Arbeitsmethoden." Vol. 4, Pg. 1352, 1935.

subcutaneous-guinea pig LDLo 100 mg/kg
"Abdernalden's Handbuch der Biologischen Arbeitsmethoden." Vol. 4, Pg. 1352, 1935.

skin-rabbit LD > 2000 mg/kg
Acute Toxicity Data. Journal of the American College of Toxicology, Part B. Vol. 12, Pg. 596, 1993.

subcutaneous-rat LDLo 404 mg/kg
Journal of Pharmacology and Experimental Therapeutics. Vol. 28, Pg. 251, 1926.

Inhalation Toxicity:
Not determined
Safety in Use Information:
buffering agents
Recommendation for guanidine HCl usage levels up to:
 not for fragrance use.
Recommendation for guanidine HCl flavor usage levels up to:
 not for flavor use.
Safety References:
EPI System: View
Daily Med:search
NIOSH International Chemical Safety Cards:search
Chemical Carcinogenesis Research Information System:Search
AIDS Citations:Search
Cancer Citations:Search
Toxicology Citations:Search
EPA Substance Registry Services (TSCA):50-01-1
EPA ACToR:Toxicology Data
EPA Substance Registry Services (SRS):Registry
Laboratory Chemical Safety Summary :5742
National Institute of Allergy and Infectious Diseases:Data
WGK Germany:1
NIST Chemistry WebBook:Search Inchi
Canada Domestic Sub. List:50-01-1
Pubchem (cid):5742
Pubchem (sid):134970848
Other Information:
(IUPAC):Atomic Weights of the Elements 2011 (pdf)
Videos:The Periodic Table of Videos
tgsc:Atomic Weights use for this web site
(IUPAC):Periodic Table of the Elements
HMDB (The Human Metabolome Database):HMDB01842
Export Tariff Code:2925.20.5000
Potential Blenders and core components note
None Found
Potential Uses:
None Found
Occurrence (nature, food, other):note
 not found in nature
 amino(imino)methanaminium chloride
 diaminomethaniminium chloride
 guanidine hydrochloride
 guanidine hydrochloride (1:1)
 guanidine monohydrochloride
 guanidine, hydrochloride (1:1)
 guanidinium hydrochloride


PubMed:Caseins from bovine colostrum and milk strongly bind piscidin-1, an antimicrobial peptide from fish.
PubMed:Synergistic effects of guanidine-grafted CMC on enhancing antimicrobial activity and dry strength of paper.
PubMed:Lipopolysaccharide induced conversion of recombinant prion protein.
PubMed:Biochemical and atomic force microscopic characterization of salmon nasal cartilage proteoglycan.
PubMed:Aggregation and adhesion properties of 22 Lactobacillus strains.
PubMed:Enhanced inhibition of foot-and-mouth disease virus by combinations of porcine interferon-α and antiviral agents.
PubMed:Akwaton, polyhexamethylene-guanidine hydrochloride-based sporicidal disinfectant: a novel tool to fight bacterial spores and nosocomial infections.
PubMed:Antimicrobial activities of polyhexamethylene guanidine hydrochloride-based disinfectant against fungi isolated from cocoa beans and reference strains of bacteria.
PubMed:Comparison of three DNA extraction methods for feed products and four amplification methods for the 5'-junction fragment of Roundup Ready soybean.
PubMed:Fluorescence studies on the stability, flexibility and substrate-induced conformational changes of acetate kinases from psychrophilic and mesophilic bacteria.
PubMed:LogSpin: a simple, economical and fast method for RNA isolation from infected or healthy plants and other eukaryotic tissues.
PubMed:Biophysical studies of an NAD(P)(+)-dependent aldehyde dehydrogenase from Bacillus licheniformis.
PubMed:Deactivation of soybean agglutinin by enzymatic and other physical treatments.
PubMed:Conformational and thermal properties of phaseolin, the major storage protein of red kidney bean (Phaseolus vulgaris L.).
PubMed:Technical note: comparing calibration methods for determination of protein in goat milk by ultraviolet spectroscopy.
PubMed:Characterization of functional intermediates of endoglucanase from Aspergillus aculeatus during urea and guanidine hydrochloride unfolding.
PubMed:Development of an optimized ELISA and a sample preparation method for the detection of beta-lactoglobulin traces in baby foods.
PubMed:Interaction of chaperone alpha-crystallin with unfolded state of alpha-amylase: Implications for reconstitution of the active enzyme.
PubMed:Identification and characterization of ambroxol as an enzyme enhancement agent for Gaucher disease.
PubMed:Methyl alpha-D-glucopyranoside enhances the enzymatic activity of recombinant beta-galactosidase inclusion bodies in the araBAD promoter system of Escherichia coli.
PubMed:Moisture-induced aggregation of whey proteins in a protein/buffer model system.
PubMed:Isolation of surface (S) layer protein carrying Lactobacillus species from porcine intestine and faeces and characterization of their adhesion properties to different host tissues.
PubMed:Effect of ionic detergents, nonionic detergents, and chaotropic agents on polyphenol oxidase activity from dormant saffron (Crocus sativus L.) corms.
PubMed:Multiplexed detection of mycotoxins in foods with a regenerable array.
PubMed:Refolding and activation of human prorenin expressed in Escherichia coli: application of recombinant human renin for inhibitor screening.
PubMed:Conformational stability of 17 beta-hydroxysteroid dehydrogenase from the fungus Cochliobolus lunatus.
PubMed:Bioanalysis and pharmacokinetics of chitosan ester in rabbit serum by HPLC with postcolumn fluorescence derivatization.
PubMed:Dimerization specificity of adult and neonatal chicken skeletal muscle myosin heavy chain rods.
PubMed:Assay of bone morphogenetic protein-2, -4, and -7 in human demineralized bone matrix.
PubMed:Modification of beta-lactoglobulin by oligofructose: impact on protein adsorption at the air-water interface.
PubMed:Rice allergenic proteins, 14-16 kDa albumin and alpha-globulin, remain insoluble in rice grains recovered from rice miso (rice-containing fermented soybean paste).
PubMed:Development of a general procedure for complete extraction of gliadins for heat processed and unheated foods.
PubMed:The production of recombinant APRP, an alkaline protease derived from Bacillus pumilus TYO-67, by in vitro refolding of pro-enzyme fixed on a solid surface.
PubMed:Study on Escherichia coli alkaline phosphatase conformation by phosphorimetry in the presence of denaturant.
PubMed:Chemical processing as a tool to generate ovalbumin variants with changed stability.
PubMed:Reversible denaturation behavior of immobilized glucose oxidase.
PubMed:A tetrodotoxin-binding protein in the hemolymph of shore crab Hemigrapsus sanguineus: purification and properties.
PubMed:Reductive unfolding and oxidative refolding of a Bowman-Birk inhibitor from horsegram seeds (Dolichos biflorus): evidence for "hyperreactive" disulfide bonds and rate-limiting nature of disulfide isomerization in folding.
PubMed:The folding of alpha-1-proteinase inhibitor: kinetic vs equilibrium control.
PubMed:Functional implications of the beta-helical protein fold: differences in chemical and thermal stabilities of Erwinia chrysanthemi EC16 pectate lyases B, C, and E.
PubMed:Structural and functional consequences of removal of the interdomain disulfide bridge from the isolated C-lobe of ovotransferrin.
PubMed:Unfolding of multimeric proteins in presence of denaturants. A case study of helianthinin from Helianthus annuus L.
PubMed:Determination of molecular weight of agars and effect of the molecular weight on the glass transition.
PubMed:Reusability of avidin-biotinylated immunoglobulin Y columns in immunoaffinity chromatography.
PubMed:Isolation and characterization of proteoglycans from growing antlers of wapiti (Cervus elaphus).
PubMed:Quantitative analysis of Gly m Bd 28K in soybean products by a sandwich enzyme-linked immunosorbent assay.
PubMed:Effects of roxarsone and monensin on digital flexoral tendons of broiler chickens.
PubMed:Assessment of the protein quality of the smooth muscle myofibrillar and connective tissue proteins of chicken gizzard.
PubMed:Status of Cys residues in the covalent structure of botulinum neurotoxin types A, B, and E.
PubMed:High-level expression of bovine beta-lactoglobulin in Pichia pastoris and characterization of its physical properties.
PubMed:Chemical and thermal stability of ferulic acid esterase-III from Aspergillus niger.
PubMed:Seminested RT-PCR systems for small round structured viruses and detection of enteric viruses in seafood.
PubMed:The detection of kinetic intermediates during the unfolding of lipoxygenase-1 by urea or guanidine hydrochloride.
PubMed:Physical, biochemical, and immunological characterization of a thermostable amidase from Klebsiella pneumoniae NCTR 1.
PubMed:Aggregated form of dextransucrases from Leuconostoc mesenteroides NRRL B-512F and its constitutive mutant.
PubMed:Role of disulfide linkages in structure and activity of proteinase inhibitor from horsegram (Dolichos biflorus).
PubMed:A comparison of methods for determining total body protein.
PubMed:Interaction of guanidine hydrochloride and guanidine thiocyanate with wheat germ lipase.
PubMed:Retrospective study of 108 cases of botulism in Poitiers, France.
PubMed:Thermostable variants of bovine beta-lactoglobulin.
PubMed:In vitro self-assembly of the S layer subunits from Clostridium difficile GAI 0714 into tetragonal arrays.
PubMed:Isolation and renaturation of bio-active proteins expressed in Escherichia coli as inclusion bodies.
PubMed:O-glycosylation and stability. Unfolding of glucoamylase induced by heat and guanidine hydrochloride.
PubMed:Active papain renatured and processed from insoluble recombinant propapain expressed in Escherichia coli.
PubMed:Molecular stability of chicken and rabbit immunoglobulin G.
PubMed:Determination of profiles of non-collagenous proteins from rat bones by sodium dodecyl sulfate high-performance liquid chromatography.
PubMed:Nature of the tryptic/chymotryptic inhibitor from horsegram (Dolichos biflorus).
PubMed:Development and characterization of a monoclonal antibody specific for Listeria monocytogenes and Listeria innocua.
PubMed:A kinetic and equilibrium study of the denaturation of aspartic proteinases from the fungi, Endothia parasitica and Mucor miehei.
PubMed:Denaturation behavior of phaseolin in urea, guanidine hydrochloride, and sodium dodecyl sulfate solutions.
PubMed:Competing solvent effects of polyols and guanidine hydrochloride on protein stability.
PubMed:Expression of recombinant bovine beta-lactoglobulin in Escherichia coli.
PubMed:Freezing denaturation of ovalbumin at acid pH.
PubMed:The binding of 3H-labelled androgen-receptor complexes to hypothalamic chromatin of neonatal mice: effect of sex and androgenization.
PubMed:Single site proteolysis in silkworm antitrypsin causes structural changes in behavior against denaturing reagents.
PubMed:Influence of glycosylation on the oligomeric structure of yeast acid phosphatase.
PubMed:Isolation and characterization of cytoplasmic fibrils from treponemes.
PubMed:Characterization and reassembly of a regular array in the cell wall of Clostridium difficile GAI 4131.
PubMed:Manganese rickets. A biochemical and stereologic study with special reference to the effect of phosphate.
PubMed:Isolation and characterization of rat intestinal polyribosomes and RNA during absorption of fat. Increased translation in vitro of apo-AIV.
PubMed:Species identification of cooked meats by enzyme-staining of isoelectricfocusing gels.
PubMed:Absorption, excretion, and metabolism of 1,3-bis(p-chlorobenzylideneamino)guanidine hydrochloride (Robenz robenidine hydrochloride) in the chicken.
PubMed:Absorption, excretion, and metabolism of robenz, robenidine hydrochloride (1,3-bis(p-chlorobenzylideneamino)guanidine hydrochloride), in the rat.
a strong organic base existing primarily as guanidium ions at physiological ph. it is found in the urine as a normal product of protein metabolism. it is also used in laboratory research as a protein denaturant. (from martindale, the extra pharmacopoeia, 30th ed and merck index, 12th ed) it is also used in the treatment of myasthenia and as a fluorescent probe in hplc.
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