| PubMed:Biocatalytic behaviour of immobilized Rhizopus oryzae lipase in the 1,3-selective ethanolysis of sunflower oil to obtain a biofuel similar to biodiesel. |
| PubMed:Quantitative Metabolomics and Instationary 13C-Metabolic Flux Analysis Reveals Impact of Recombinant Protein Production on Trehalose and Energy Metabolism in Pichia pastoris. |
| PubMed:Monoolein production by triglycerides hydrolysis using immobilized Rhizopus oryzae lipase. |
| PubMed:Genome sequence and transcriptome analyses of the thermophilic zygomycete fungus Rhizomucor miehei. |
| PubMed:Eco-friendly methodology for efficient synthesis and scale-up of 2-ethylhexyl-p-methoxycinnamate using Rhizopus oryzae lipase and its biological evaluation. |
| PubMed:Comprehensive clone screening and evaluation of fed-batch strategies in a microbioreactor and lab scale stirred tank bioreactor system: application on Pichia pastoris producing Rhizopus oryzae lipase. |
| PubMed:Study of different reaction schemes for the enzymatic synthesis of polyglycerol polyricinoleate. |
| PubMed:High-level expression of pro-form lipase from Rhizopus oryzae in Pichia pastoris and its purification and characterization. |
| PubMed:Optimization of marine waste based-growth media for microbial lipase production using mixture design methodology. |
| PubMed:Organic synthesis of maize starch-based polymer using Rhizopus oryzae lipase, scale up, and its characterization. |
| PubMed:Biodiesel production in a magnetically-stabilized, fluidized bed reactor with an immobilized lipase in magnetic chitosan microspheres. |
| PubMed:Metabolic flux analysis of recombinant Pichia pastoris growing on different glycerol/methanol mixtures by iterative fitting of NMR-derived (13)C-labelling data from proteinogenic amino acids. |
| PubMed:Solvent-Free Synthesis of Flavour Esters through Immobilized Lipase Mediated Transesterification. |
| PubMed:Lipases efficiently stearate and cutinases acetylate the surface of arabinoxylan films. |
| PubMed:Role of Met93 and Thr96 in the lid hinge region of Rhizopus chinensis lipase. |
| PubMed:High-level expression and characterization of a chimeric lipase from Rhizopus oryzae for biodiesel production. |
| PubMed:Assessment of activities and conformation of lipases treated with sub- and supercritical carbon dioxide. |
| PubMed:Esterification of polyglycerol with polycondensed ricinoleic acid catalysed by immobilised Rhizopus oryzae lipase. |
| PubMed:Enzymatic transesterification of palm stearin and olein blends to produce zero-trans margarine fat. |
| PubMed:Synthesis of 2-monoacylglycerols and structured triacylglycerols rich in polyunsaturated fatty acids by enzyme catalyzed reactions. |
| PubMed:Immobilization of lipase from Mucor miehei and Rhizopus oryzae into mesoporous silica--the effect of varied particle size and morphology. |
| PubMed:Crosslinked aggregates of Rhizopus oryzae lipase as industrial biocatalysts: preparation, optimization, characterization, and application for enantioselective resolution reactions. |
| PubMed:Efficient heterologous expression and secretion in Aspergillus oryzae of a llama variable heavy-chain antibody fragment V(HH) against EGFR. |
| PubMed:A simple and accurate two-step long DNA sequences synthesis strategy to improve heterologous gene expression in pichia. |
| PubMed:Metabolic flux profiling of recombinant protein secreting Pichia pastoris growing on glucose:methanol mixtures. |
| PubMed:Comparative analysis for the production of fatty acid alkyl esterase using whole cell biocatalyst and purified enzyme from Rhizopus oryzae on waste cooking oil (sunflower oil). |
| PubMed:Codon optimization, expression and enzymatic comparison of Rhizopus oryzae lipases pro-ROL and m-ROL in Pichia pastoris. |
| PubMed:Heterologous expression systems for lipases: a review. |
| PubMed:Characterization of smallest active monomeric lipase from novel rhizopus strain: application in transesterification. |
| PubMed:Production of recombinant Rhizopus oryzae lipase by the yeast Yarrowia lipolytica results in increased enzymatic thermostability. |
| PubMed:Water activity dependence of performance of surface-displayed lipase in yeast cells: a unique water requirement for enzymatic synthetic reaction in organic media. |
| PubMed:Improved activity and stability of Rhizopus oryzae lipase via immobilization for citronellol ester synthesis in supercritical carbon dioxide. |
| PubMed:Expression and characterization of recombinant Rhizopus oryzae lipase for enzymatic biodiesel production. |
| PubMed:Construction of the yeast whole-cell Rhizopus oryzae lipase biocatalyst with high activity. |
| PubMed:Fungi isolated from olive ecosystems and screening of their potential biotechnological use. |
| PubMed:Immobilized Rhizopus oryzae lipase catalyzed synthesis of palm stearin and cetyl alcohol wax esters: optimization by response surface methodology. |
| PubMed:Immobilization and stability of a Rhizopus oryzae lipase expressed in Pichia pastoris: comparison between native and recombinant variants. |
| PubMed:Transesterification of phosphatidylcholine in sn-1 position through direct use of lipase-producing Rhizopus oryzae cells as whole-cell biocatalyst. |
| PubMed:Mechanism of acetaldehyde-induced deactivation of microbial lipases. |
| PubMed:Isolation, identification and optimization of a new extracellular lipase producing strain of Rhizopus sp. |
| PubMed:A large scale enzyme screen in the search for new methods of silicon-oxygen bond formation. |
| PubMed:Enantioselective production of benzoin from benzoin acetate via kinetic resolution and deracemization using Rhizopus oryzae. |
| PubMed:Biodiesel production by a mixture of Candida rugosa and Rhizopus oryzae lipases using a supercritical carbon dioxide process. |
| PubMed:Enzyme mediated silicon-oxygen bond formation; the use of Rhizopus oryzae lipase, lysozyme and phytase under mild conditions. |
| PubMed:Surfactant-modified yeast whole-cell biocatalyst displaying lipase on cell surface for enzymatic production of structured lipids in organic media. |
| PubMed:Strategies for utilisation of food-processing wastes to produce lipases in solid-state cultures of Rhizopus oryzae. |
| PubMed:Structural homologies, importance for catalysis and lipid binding of the N-terminal peptide of a fungal and a pancreatic lipase. |
| PubMed:Significant changes in the transesterification activity of free and mesoporous-immobilized Rhizopus oryzae lipase in ionic liquids. |
| PubMed:[Stability of whole cell biocatalyst for biodiesel production from renewable oils]. |
| PubMed:Preparation of (S)-1-Halo-2-octanols Using Ionic Liquids and Biocatalysts. |
| PubMed:Development of batch and continuous processes on biodiesel production in a packed-bed reactor by a mixture of immobilized Candida rugosa and Rhizopus oryzae lipases. |
| PubMed:Optimization of biodiesel production catalyzed by fungus cells immobilized in fibrous supports. |
| PubMed:Engineering of bottlenecks in Rhizopus oryzae lipase production in Pichia pastoris using the nitrogen source-regulated FLD1 promoter. |
| PubMed:Water activity dependence of lipases in non-aqueous biocatalysis. |
| PubMed:Effect of a buffer mixture system on the activity of lipases during immobilization process. |
| PubMed:Screening of food grade lipases to be used in esterification and interesterification reactions of industrial interest. |
| PubMed:Optimization of the process for biodiesel production using a mixture of immobilized Rhizopus oryzae and Candida rugosa lipases. |
| PubMed:Preparation and comparative characterization of immobilized Aspergillus oryzae expressing Fusarium heterosporum lipase for enzymatic biodiesel production. |
| PubMed:Physical immobilization of Rhizopus oryzae lipase onto cellulose substrate: activity and stability studies. |
| PubMed:Role of N-terminal 28-amino-acid region of Rhizopus oryzae lipase in directing proteins to secretory pathway of Aspergillus oryzae. |
| PubMed:Optimization of lipase pretreatment prior to lipase immobilization to prevent loss of activity. |
| PubMed:Enhancement of the stability of a prolipase from Rhizopus oryzae toward aldehydes by saturation mutagenesis. |
| PubMed:Comparative study of thermostability and ester synthesis ability of free and immobilized lipases on cross linked silica gel. |
| PubMed:Transcriptional response of P. pastoris in fed-batch cultivations to Rhizopus oryzae lipase production reveals UPR induction. |
| PubMed:Production of a Rhizopus oryzae lipase from Pichia pastoris using alternative operational strategies. |
| PubMed:Rivoflavin may interfere with on-line monitoring of secreted green fluorescence protein fusion proteins in Pichia pastoris. |
| PubMed:Production of butyl acetate ester by lipase from novel strain of Rhizopus oryzae. |
| PubMed:Sorbitol co-feeding reduces metabolic burden caused by the overexpression of a Rhizopus oryzae lipase in Pichia pastoris. |
| PubMed:Construction of a novel synergistic system for production and recovery of secreted recombinant proteins by the cell surface engineering. |
| PubMed:Preparation of a whole-cell biocatalyst of mutated Candida antarctica lipase B (mCALB) by a yeast molecular display system and its practical properties. |
| PubMed:Combining regio- and enantioselectivity of lipases for the preparation of (R)-4-chloro-2-butanol. |
| PubMed:Cloning, disruption and protein secretory phenotype of the GAS1 homologue of Pichia pastoris. |
| PubMed:Enhancement of activity of lipase-displaying yeast cells and their application to optical resolution of (R,S)-1-benzyloxy-3-chloro-2-propyl monosuccinate. |
| PubMed:Construction of a Pichia pastoris cell-surface display system using Flo1p anchor system. |
| PubMed:Biochemical and molecular characterization of a lipase produced by Rhizopus oryzae. |
| PubMed:Plasmids for expression of heterologous proteins in Rhizopus oryzae. |
| PubMed:A simple model-based control for Pichia pastoris allows a more efficient heterologous protein production bioprocess. |
| PubMed:Lipase localization in Rhizopus oryzae cells immobilized within biomass support particles for use as whole-cell biocatalysts in biodiesel-fuel production. |
| PubMed:Lipase-catalyzed esterification of 2-monoricinolein for 1,2 (2,3)-diricinolein synthesis. |
| PubMed:State variables monitoring by in situ multi-wavelength fluorescence spectroscopy in heterologous protein production by Pichia pastoris. |
| PubMed:Heterologous production of functional forms of Rhizopus oryzae lipase in Escherichia coli. |
| PubMed:Olive-mill wastewaters: a promising substrate for microbial lipase production. |
| PubMed:Cell surface engineering of yeast: construction of arming yeast with biocatalyst. |
| PubMed:Biodiesel fuel production from plant oil catalyzed by Rhizopus oryzae lipase in a water-containing system without an organic solvent. |
| PubMed:Enhanced reactivity of Rhizopus oryzae lipase displayed on yeast cell surfaces in organic solvents: potential as a whole-cell biocatalyst in organic solvents. |
| PubMed:Developing high cell density fed-batch cultivation strategies for heterologous protein production in Pichia pastoris using the nitrogen source-regulated FLD1 Promoter. |
| PubMed:Combined effect of the methanol utilization (Mut) phenotype and gene dosage on recombinant protein production in Pichia pastoris fed-batch cultures. |
| PubMed:Creation of Rhizopus oryzae lipase having a unique oxyanion hole by combinatorial mutagenesis in the lid domain. |
| PubMed:N-terminal peptide of Rhizopus oryzae lipase is important for its catalytic properties. |
| PubMed:Model studies on the formation of monochloropropanediols in the presence of lipase. |
| PubMed:Use of immobilized lipases for lipase purification via specific lipase-lipase interactions. |
| PubMed:Expression of a Rhizopus oryzae lipase in Pichia pastoris under control of the nitrogen source-regulated formaldehyde dehydrogenase promoter. |
| PubMed:Enantioselective transesterification using lipase-displaying yeast whole-cell biocatalyst. |
| PubMed:Fluorescent organophosphonates as inhibitors of microbial lipases. |
| PubMed:Evaluation of the catalytic activity of lipases immobilized on chrysotile for esterification. |
| PubMed:Endophytic fungi associated with Mediterranean plants as a source of mycelium-bound lipases. |
| PubMed:Safety evaluation of lipase produced from Rhizopus oryzae: summary of toxicological data. |
| PubMed:Construction of yeast strains with high cell surface lipase activity by using novel display systems based on the Flo1p flocculation functional domain. |
| PubMed:Gas-phase enzymatic esterification on immobilized lipases in MCM-41 molecular sieves. |
| PubMed:Fluorescent inhibitors reveal solvent-dependent micropolarity in the lipid binding sites of lipases. |
| PubMed:Synthesis of phosphatidylcholine with defined fatty acid in the sn-1 position by lipase-catalyzed esterification and transesterification reaction. |
| PubMed:Yeast whole-cell biocatalyst constructed by intracellular overproduction of Rhizopus oryzae lipase is applicable to biodiesel fuel production. |
| PubMed:Lipase-catalysed hydrolysis of short-chain substrates in solution and in emulsion: a kinetic study. |
| PubMed:Spacer-mediated display of active lipase on the yeast cell surface. |
| PubMed:Kinetic studies of Rhizopus oryzae lipase using monomolecular film technique. |
| PubMed:Function of the prosequence for in vivo folding and secretion of active Rhizopus oryzae lipase in Saccharomyces cerevisiae. |
| PubMed:Whole cell biocatalyst for biodiesel fuel production utilizing Rhizopus oryzae cells immobilized within biomass support particles. |
| PubMed:Optimization of the high-level production of Rhizopus oryzae lipase in Pichia pastoris. |
| PubMed:The His gap motif in microbial lipases: a determinant of stereoselectivity toward triacylglycerols and analogs. |
| PubMed:Purification and characterization of an extracellular lipase from a thermophilic Rhizopus oryzae strain isolated from palm fruit. |
| PubMed:Independent production of two molecular forms of a recombinant Rhizopus oryzae lipase by KEX2-engineered strains of Saccharomyces cerevisiae. |
| PubMed:Molecular dynamics of microbial lipases as determined from their intrinsic tryptophan fluorescence. |
| PubMed:Interactions of fluorescent triacylglycerol analogs covalently bound to the active site of a lipase from Rhizopus oryzae. |
| PubMed:A toolbox of recombinant lipases for industrial applications. |
| PubMed:Functional expression of Rhizopus oryzae lipase in Pichia pastoris: high-level production and some properties. |
| PubMed:Cloning, expression, characterization and role of the leader sequence of a lipase from Rhizopus oryzae. |
| PubMed:Rational design of Rhizopus oryzae lipase with modified stereoselectivity toward triradylglycerols. |
| PubMed:Inhibition of lipases from Chromobacterium viscosum and Rhizopus oryzae by tetrahydrolipstatin. |
| PubMed:Safety evaluation of lipase derived from Rhizopus oryzae: summary of toxicological data. |
| PubMed:Inhibition of microbial lipases with stereoisomeric triradylglycerol analog phosphonates. |
| PubMed:The folding and activity of the extracellular lipase of Rhizopus oryzae are modulated by a prosequence. |
| PubMed:Analysis of the catalytic mechanism of a fungal lipase using computer-aided design and structural mutants. |
| PubMed:Interesterification of butter fat by partially purified extracellular lipases from Pseudomonas putida, Aspergillus niger and Rhizopus oryzae. |
| PubMed:[Premedication for sonography. Enzyme-paractol in comparison with a placebo]. |
| PubMed:[Immobilized lipase from Rhizopus oryzae 14-14]. |
| PubMed:[Experiences with substitution therapy using a new pancreatic enzyme of plant origin]. |