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polygalacturonase
polygalacturonase from aspergillus niger

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CAS Number: 9032-75-1
Other(deleted CASRN):107397-63-7
ECHA EC Number:232-885-6
FDA UNII: Search
Category:enzyme preparations and microorganisms
 
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 FDA/DG SANTE Petitions, Reviews, Notices:
GRN 89 Five enzyme preparations from Aspergillus niger: Carbohydrase enzyme preparation, catalase enzyme preparation, glucose oxidase enzyme preparation, pectinase enzyme preparation, and protease enzyme preparation View - notice PDF
GRN 557 Polygalacturonase from Aspergillus tubingensis expressed in Trichoderma reesei View - notice PDF
JECFA Food Additive: Pectinase from Aspergillus niger, var.
FDA Mainterm (SATF):977031-85-8 ; PECTINASE FROM ASPERGILLUS NIGER
 
Physical Properties:
Appearance:pale tan to dark brown powder to liquid (est)
Food Chemicals Codex Listed: No
Soluble in:
 water
Insoluble in:
 alcohol
 ether
 
Organoleptic Properties:
Odor and/or flavor descriptions from others (if found).
 
Cosmetic Information:
None found
 
Suppliers:
BOC Sciences
For experimental / research use only.
Pectinase
Charkit Chemical
PECTINASE
Shaanxi Y-Herb Biotechnology
99% Purity Pectinase Enzyme Cellulase Powder 9032-75-1 Fruits Processing Use
Shaanxi Y-Herb Biotechnology
Food Grade Pectinase Enzyme Powder Supplement 30000u/g For Fruit Juice
 
Safety Information:
 
Hazards identification
 
Classification of the substance or mixture
GHS Classification in accordance with 29 CFR 1910 (OSHA HCS)
None found.
GHS Label elements, including precautionary statements
 
Pictogram
 
Hazard statement(s)
None found.
Precautionary statement(s)
None found.
Oral/Parenteral Toxicity:
Not determined
Dermal Toxicity:
Not determined
Inhalation Toxicity:
Not determined
 
Safety in Use Information:
Category:
enzyme preparations and microorganisms
Recommendation for polygalacturonase usage levels up to:
 not for fragrance use.
 
Recommendation for polygalacturonase flavor usage levels up to:
 not for flavor use.
 
Safety References:
European Food Safety Authority (EFSA) reference(s):

Annual report on the preparatory work for the evaluation of food enzymes – Toxicological part
View page or View pdf

Safety and efficacy of FRA® Octazyme C Dry(endo-1,4-ß-xylanase, mannan-endo-1,4-ß-mannosidase, a-amylase, endo-1,3(4)-ß-glucanase, pectinase, endo-1,4-ß-glucanase, protease, a-galactosidase) as a feed additive for weaned piglets and chickens for fattening
View page or View pdf

EPA Substance Registry Services (TSCA):9032-75-1
EPA ACToR:Toxicology Data
EPA Substance Registry Services (SRS):Registry
National Institute of Allergy and Infectious Diseases:Data
Chemidplus:0009032751
 
References:
Pubchem (sid):135309565
 
Other Information:
FDA Substances Added to Food (formerly EAFUS):View
HMDB (The Human Metabolome Database):Search
FooDB:FDB020174
FDA Listing of Food Additive Status:View
VCF-Online:VCF Volatile Compounds in Food
Wikipedia:View
 
Potential Blenders and core components note
None Found
 
Potential Uses:
None Found
 
Occurrence (nature, food, other):note
 apple plant
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 aspergillus niger
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 burdock leaf
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 pectinase
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 tomato fruit
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Synonyms:
 pectinase from aspergillus niger
 polygalacturonase from aspergillus niger
 polygalacturonase from aspergillus tubingensis expressed in trichoderma reesei
 

Articles:

PubMed:Secretory expression and characterization of a acidic endo-polygalacturonase gene from Aspergillus niger SC323 in Saccharomyces cerevisiae.
PubMed:Immuno-affinity purification of PglPGIP1, a polygalacturonase-inhibitor protein from pearl millet: studies on its inhibition of fungal polygalacturonases and role in resistance against the downy mildew pathogen.
PubMed:Biochemical Characterization of a Novel Endo-1,5-α-l-arabinanase from Rhizomucor miehei.
PubMed:Production and immobilization of enzymes by solid-state fermentation of agroindustrial waste.
PubMed:Controlled expression of pectic enzymes in Arabidopsis thaliana enhances biomass conversion without adverse effects on growth.
PubMed:Experimental and bioinformatic characterization of a recombinant polygalacturonase-inhibitor protein from pearl millet and its interaction with fungal polygalacturonases.
PubMed:Production and characterization of in planta transiently produced polygalacturanase from Aspergillus niger and its fusions with hydrophobin or ELP tags.
PubMed:Aspergillus niger RhaR, a regulator involved in L-rhamnose release and catabolism.
PubMed:Optimized production of vanillin from green vanilla pods by enzyme-assisted extraction combined with pre-freezing and thawing.
PubMed:Cold active pectinases: advancing the food industry to the next generation.
PubMed:Production of plant cell wall degrading enzymes by monoculture and co-culture of Aspergillus niger and Aspergillus terreus under SSF of banana peels.
PubMed:Fungal endopolygalacturonases are recognized as microbe-associated molecular patterns by the arabidopsis receptor-like protein RESPONSIVENESS TO BOTRYTIS POLYGALACTURONASES1.
PubMed:Cloning, expression of Aspergillus niger JL-15 endo-polygalacturonase A gene in Pichia pastoris and oligo-galacturonates production.
PubMed:The capability of endophytic fungi for production of hemicellulases and related enzymes.
PubMed:Extraction of DNA from orange juice, and detection of bacterium Candidatus Liberibacter asiaticus by real-time PCR.
PubMed:Optimization of enzymatic treatment for compound K production from white ginseng extract by response surface methodology.
PubMed:Evaluation of free and immobilized Aspergillus niger NRC1ami pectinase applicable in industrial processes.
PubMed:Production of polygalacturonases by Aspergillus section Nigri strains in a fixed bed reactor.
PubMed:Enzymatic depolymerization of gum tragacanth: bifidogenic potential of low molecular weight oligosaccharides.
PubMed:Purification and physicochemical properties of polygalacturonase from Aspergillus niger MTCC 3323.
PubMed:Improvement of tea leaves fermentation through pectinases.
PubMed:A novel polygalacturonase-inhibiting protein (PGIP) from Lathyrus sativus L. seeds.
PubMed:Polygalacturonase-inhibitor proteins in pearl millet: possible involvement in resistance against downy mildew.
PubMed:Bioresponsive systems based on polygalacturonate containing hydrogels.
PubMed:Enzyme-assisted extraction of flavonoids from Ginkgo biloba leaves: improvement effect of flavonol transglycosylation catalyzed by Penicillium decumbens cellulase.
PubMed:Definition and characterization of enzymes for maximal biocatalytic solubilization of prebiotic polysaccharides from potato pulp.
PubMed:Efficacy of xylanase purified from Aspergillus niger DFR-5 alone and in combination with pectinase and cellulase to improve yield and clarity of pineapple juice.
PubMed:The behavior of kinetic parameters in production of pectinase and xylanase by solid-state fermentation.
PubMed:Improvement of polygalacturonase production at high temperature by mixed culture of Aspergillus niger and Saccharomyces cerevisiae.
PubMed:Fungi isolated from olive ecosystems and screening of their potential biotechnological use.
PubMed:Aspergillus carbonarius polygalacturonases purified by integrated membrane process and affinity precipitation for apple juice production.
PubMed:Isolation of intact sub-dermal secretory cavities from Eucalyptus.
PubMed:Screening of pectinase-producing microorganisms with polygalacturonase activity.
PubMed:Engineering the cell wall by reducing de-methyl-esterified homogalacturonan improves saccharification of plant tissues for bioconversion.
PubMed:Three aspartic acid residues of polygalacturonase-inhibiting protein (PGIP) from Phaseolus vulgaris are critical for inhibition of Fusarium phyllophilum PG.
PubMed:Simultaneous non-thermal saccharification of cassava pulp by multi-enzyme activity and ethanol fermentation by Candida tropicalis.
PubMed:Substrate specificities of glycosidases from Aspergillus species pectinase preparations on elderberry anthocyanins.
PubMed:Endopolygalacturonases reveal molecular features for processivity pattern and tolerance towards acetylated pectin.
PubMed:Agricultural waste from the tequila industry as substrate for the production of commercially important enzymes.
PubMed:Synthesis of glyceryl ferulate by immobilized ferulic acid esterase.
PubMed:Immobilization of polygalacturonase from Aspergillus niger onto activated polyethylene and its application in apple juice clarification.
PubMed:Characterization of an exopolygalacturonase from Aspergillus niger.
PubMed:Evidence for a blockwise distribution of acetyl groups onto homogalacturonans from a commercial sugar beet (Beta vulgaris) pectin.
PubMed:Overproduction of laccase and pectinase by microbial associations in solid substrate fermentation.
PubMed:Crystal structure of Aspergillus niger isopullulanase, a member of glycoside hydrolase family 49.
PubMed:Transgenic expression of a fungal endo-polygalacturonase increases plant resistance to pathogens and reduces auxin sensitivity.
PubMed:The heterologous expression of polysaccharidase-encoding genes with oenological relevance in Saccharomyces cerevisiae.
PubMed:Enzymes with new biochemical properties in the pectinolytic complex produced by Aspergillus niger MIUG 16.
PubMed:Fungal multienzyme production on industrial by-products of the citrus-processing industry.
PubMed:Optimization of cellulase complex formulation for peashrub biomass hydrolysis.
PubMed:Digital image processing as a tool to monitor biomass growth in Aspergillus niger 3T5B8 solid-state fermentation: preliminary results.
PubMed:Enzyme-catalyzed change of antioxidants content and antioxidant activity of asparagus juice.
PubMed:The grapevine polygalacturonase-inhibiting protein (VvPGIP1) reduces Botrytis cinerea susceptibility in transgenic tobacco and differentially inhibits fungal polygalacturonases.
PubMed:On the simulation of enzymatic digest patterns: the fragmentation of oligomeric and polymeric galacturonides by endo-polygalacturonase II.
PubMed:Protease-assisted clarification of black currant juice: synergy with other clarifying agents and effects on the phenol content.
PubMed:Glycan analysis of recombinant Aspergillus niger endo-polygalacturonase A.
PubMed:Optimization of multienzyme production by two mixed strains in solid-state fermentation.
PubMed:Esterification of ferulic acid with polyols using a ferulic acid esterase from Aspergillus niger.
PubMed:Comprehensive glycan analysis of recombinant Aspergillus niger endo-polygalacturonase C.
PubMed:The effect of polysaccharide-degrading wine yeast transformants on the efficiency of wine processing and wine flavour.
PubMed:Pectinase production by solid fermentation from Aspergillus niger by a new prescription experiment.
PubMed:Apple polygalacturonase inhibiting protein1 expressed in transgenic tobacco inhibits polygalacturonases from fungal pathogens of apple and the anthracnose pathogen of lupins.
PubMed:Optimization of process for the production of fungal pectinases from deseeded sunflower head in submerged and solid-state conditions.
PubMed:Production of pectinase from deseeded sunflower head by Aspergillus niger in submerged and solid-state conditions.
PubMed:[Study on breeding of probiotic Aspergillus nigre by N+ implantation and fermentation condition of its mutant].
PubMed:The contribution of ionic interactions to the conformational stability and function of polygalacturonase from A. niger.
PubMed:Influence of pectin and glucose on growth and polygalacturonase production by Aspergillus niger in solid-state cultivation.
PubMed:The biocompatibility of dibutyryl chitin in the context of wound dressings.
PubMed:Action pattern of Fusarium moniliforme endopolygalacturonase towards pectin fragments: Comprehension and prediction.
PubMed:Chitooligosaccharides--preparation with the aid of pectinase isozyme from Aspergillus niger and their antibacterial activity.
PubMed:Effect of pectolytic enzyme preparations on the phenolic composition and antioxidant activity of asparagus juice.
PubMed:Depolymerization of beta-chitin to mono- and disaccharides by the serum fraction from the para rubber tree, Hevea brasiliensis.
PubMed:Evaluation of microbeads of calcium alginate as a fluidized bed medium for affinity chromatography of Aspergillus niger Pectinase.
PubMed:Polygalacturonase-inhibiting proteins can function as activators of polygalacturonase.
PubMed:Targeted modification of homogalacturonan by transgenic expression of a fungal polygalacturonase alters plant growth.
PubMed:Pectinase production by Aspergillus niger using wastewater in solid state fermentation for eliciting plant disease resistance.
PubMed:The OsFOR1 gene encodes a polygalacturonase-inhibiting protein (PGIP) that regulates floral organ number in rice.
PubMed:Affinity precipitation of Aspergillus niger pectinase by microwave-treated alginate.
PubMed:Structural insights into the processivity of endopolygalacturonase I from Aspergillus niger.
PubMed:Low molecular weight chitosans--preparation by depolymerization with Aspergillus niger pectinase, and characterization.
PubMed:Chemically methylated and reduced pectins: preparation, characterisation by 1H NMR spectroscopy, enzymatic degradation, and gelling properties.
PubMed:Elucidation of pectin methylester distributions by capillary electrophoresis.
PubMed:Regulation of the production of polygalacturonase by Aspergillus niger.
PubMed:Potential use of cutinase in enzymatic scouring of cotton fiber cuticle.
PubMed:Evaluation of filamentous fungi and inducers for the production of endo-polygalacturonase by solid state fermentation.
PubMed:Purification and characterisation of two exo-polygalacturonases from Aspergillus niger able to degrade xylogalacturonan and acetylated homogalacturonan.
PubMed:Studying protein-carbohydrate interactions by amide hydrogen/deuterium exchange mass spectrometry.
PubMed:Use of amide exchange mass spectrometry to study conformational changes within the endopolygalacturonase II-homogalacturonan-polygalacturonase inhibiting protein system.
PubMed:A novel enzyme activity involving the demethylation of specific partially methylated oligogalacturonides.
PubMed:Flax-retting by polygalacturonase-containing enzyme mixtures and effects on fiber properties.
PubMed:SCP and crude pectinase production by slurry-state fermentation of lemon pulps.
PubMed:A simple fractionation protocol for, and a comprehensive study of the molecular properties of, two major endopolygalacturonases from Aspergillus niger.
PubMed:On-target endoglycosidase digestion matrix-assisted laser desorption/ionization mass spectrometry of glycopeptides.
PubMed:Pectin degrading glycoside hydrolases of family 28: sequence-structural features, specificities and evolution.
PubMed:The X-ray structure of Aspergillus aculeatus polygalacturonase and a modeled structure of the polygalacturonase-octagalacturonate complex.
PubMed:Exopectinases produced by Aspergillus niger in solid-state and submerged fermentation: a comparative study.
PubMed:Changing a single amino acid residue switches processive and non-processive behavior of Aspergillus niger endopolygalacturonase I and II.
PubMed:Selective and sensitive detection of pectin lyase activity using a colorimetric test: application to the screening of microorganisms possessing pectin lyase activity.
PubMed:Inhibition of polygalacturonase from Verticillium dahliae by a polygalacturonase inhibiting protein from cotton.
PubMed:Comparison of the efficacies of a novel aspergillus niger mycelium with separate and combined effectiveness of phytase, acid phosphatase, and pectinase in dephosphorylation of wheat-based feeds fed to growing broilers.
PubMed:Quantification of the amount of galacturonic acid residues in blocksequences in pectin homogalacturonan by enzymatic fingerprinting with exo- and endo-polygalacturonase II from Aspergillus niger.
PubMed:Analysis of different de-esterification mechanisms for pectin by enzymatic fingerprinting using endopectin lyase and endopolygalacturonase II from A. niger.
PubMed:Polygalacturonase is the key component in enzymatic retting of flax.
PubMed:Enzyme, bacterial inoculant, and formic acid effects on silage composition of orchardgrass and alfalfa.
PubMed:Subsite mapping of Aspergillus niger endopolygalacturonase II by site-directed mutagenesis.
PubMed:Characterization of a novel endopolygalacturonase from Aspergillus niger with unique kinetic properties.
PubMed:pgaA and pgaB encode two constitutively expressed endopolygalacturonases of Aspergillus niger.
PubMed:The active site topology of Aspergillus niger endopolygalacturonase II as studied by site-directed mutagenesis.
PubMed:Performance of selected microbial pectinases on synthetic monomethyl-esterified di- and trigalacturonates.
PubMed:Genetic improvement of Aspergillus carbonarius for pectinase overproduction during solid state growth.
PubMed:1.68-A crystal structure of endopolygalacturonase II from Aspergillus niger and identification of active site residues by site-directed mutagenesis.
PubMed:Characterization of the glycosylation of recombinant endopolygalacturonase I from Aspergillus niger.
PubMed:The specificity of polygalacturonase-inhibiting protein (PGIP): a single amino acid substitution in the solvent-exposed beta-strand/beta-turn region of the leucine-rich repeats (LRRs) confers a new recognition capability.
PubMed:Sequencing of partially methyl-esterified oligogalacturonates by tandem mass spectrometry and its use to determine pectinase specificities.
PubMed:Kinetic characterization of Aspergillus niger N400 endopolygalacturonases I, II and C.
PubMed:The endopolygalacturonase gene Bcpg1 is required for full virulence of Botrytis cinerea.
PubMed:Batch foam fractionation of kudzu (Pueraria lobata) vine retting solution.
PubMed:The glycoprotein character of multiple forms of Aspergillus polygalacturonase.
PubMed:Production of pectinesterase and polygalacturonase by Aspergillus niger in submerged and solid state systems.
PubMed:pgaE encodes a fourth member of the endopolygalacturonase gene family from Aspergillus niger.
PubMed:Polygalacturonase-inhibiting proteins (PGIPs) with different specificities are expressed in Phaseolus vulgaris.
PubMed:Analysis of conductance responses during depolymerization of pectate by soft rot Erwinia spp. and other pectolytic bacteria isolated from potato tubers.
PubMed:Production of Aspergillus niger pectolytic enzymes by solid state bioprocessing of apple pomace.
PubMed:The filamentous fungus Aspergillus niger contains two "differentially regulated" trehalose-6-phosphate synthase-encoding genes, tpsA and tpsB.
PubMed:Identification of the glycosylation site and glycan structures of recombinant endopolygalacturonase II by mass spectrometry.
PubMed:Release of ferulic acid from sugar-beet pulp by using arabinanase, arabinofuranosidase and an esterase from Aspergillus niger.
PubMed:Production of pectinases by Aspergillus niger in solid state fermentation at high initial glucose concentrations.
PubMed:Expression of an Erwinia pectate lyase in three species of Aspergillus.
PubMed:Inversion of configuration during hydrolysis of alpha-1,4-galacturonidic linkage by three Aspergillus polygalacturonases.
PubMed:The efficacy of an enzymic cocktail and a fungal mycelium in dephosphorylating corn-soybean meal-based feeds fed to growing turkeys.
PubMed:Efficient expression and secretion of Aspergillus niger RH5344 polygalacturonase in Saccharomyces cerevisiae.
PubMed:Cloning, sequence and expression of the gene coding for rhamnogalacturonase of Aspergillus aculeatus; a novel pectinolytic enzyme.
PubMed:Production and properties of three pectinolytic activities produced by Aspergillus niger in submerged and solid-state fermentation.
PubMed:Isolation and characterization of polygalacturonase genes (pecA and pecB) from Aspergillus flavus.
PubMed:Substrate binding ability of chemically inactivated pectinase for the substrate pectic acid.
PubMed:Cry j 2, a major allergen of Japanese cedar pollen, shows polymethylgalacturonase activity.
PubMed:Seeds as natural matrices for immobilization of Aspergillus niger mycelium producing pectinases.
PubMed:Immobilization of Aspergillus niger xylanase on magnetic latex beads.
PubMed:Cloning and characterization of a novel polygalacturonase-encoding gene from Aspergillus parasiticus.
PubMed:Gene expression in filamentous fungi. Expression of pectinases and glucose oxidase in Aspergillus niger.
PubMed:Crystallization and preliminary crystallographic characterization of endo-polygalacturonase II from Aspergillus niger.
PubMed:Purification and molecular characterization of a soybean polygalacturonase-inhibiting protein.
PubMed:Purification of endo-polygalacturonase by affinity precipitation using alginate.
PubMed:Structural features of a polygalacturonase gene cloned from Aspergillus oryzae KBN616.
PubMed:Ferulic acid esterase from Aspergillus niger: purification and partial characterization of two forms from a commercial source of pectinase.
PubMed:Methanol utilization by a strain of Aspergillus niger: influence on the synthesis and activity of pectinases.
PubMed:New approach for selecting pectinase producing mutants of Aspergillus niger well adapted to solid state fermentation.
PubMed:Characterization of endopolygalacturonase (EC 3.2.1.15) from Aspergillus niger as glycoprotein by electrophoretic methods and lectin affino-blotting.
PubMed:The polygalacturonases of Aspergillus niger are encoded by a family of diverged genes.
PubMed:Identification and characterization of a second polygalacturonase gene of Aspergillus niger.
PubMed:Expression and sequence comparison of the Aspergillus niger and Aspergillus tubigensis genes encoding polygalacturonase II.
PubMed:Characterization of a polygalacturonase gene of Aspergillus niger RH5344.
PubMed:Purification and characterization of two endopolygalacturonases from Sclerotinia sclerotiorum.
PubMed:Synthesis of different pectinases by filamentous growing A. niger mutants.
PubMed:Molecular cloning, nucleotide sequence and expression of the gene encoding prepro-polygalacturonaseII of Aspergillus niger.
PubMed:Cloning and DNA sequence analysis of a polygalacturonase cDNA from Aspergillus niger RH5344.
PubMed:Common amino acid domain among endopolygalacturonases of ascomycete fungi.
PubMed:Cloning and expression of a second Aspergillus niger pectin lyase gene (pelA): indications of a pectin lyase gene family in A. niger.
PubMed:Purification and characterization of polygalacturonases produced by the hyphal fungus Aspergillus niger.
PubMed:Immobilization of endo-polygalacturonase from Aspergillus niger on various types of macromolecular supports.
PubMed:Activity stain for polygalacturonase.
PubMed:Purification and Characterization of a Polygalacturonase-Inhibiting Protein from Phaseolus vulgaris L.
PubMed:Elicitation of Necrosis in Vigna unguiculata Walp. by Homogeneous Aspergillus niger Endo-Polygalacturonase and by alpha-d-Galacturonate Oligomers.
PubMed:Influence of culture conditions on mycelial structure and polygalacturonase synthesis of Aspergillus niger.
PubMed:Pectinolytic activity of revertants of auxotrophic strains of Aspergillus niger.
PubMed:New matrices for the purification of pectinases by affinity chromatography.
PubMed:[The distribution of free and esterified carboxyl groups within the pectin molecule after the action of pectin esterase from Aspergillus niger and oranges].
PubMed:A fungal polygalacturonase activating factor from tomato fruit.
PubMed:Selective isolation of endo-D-galacturonanase of Aspergillus niger based on interaction with tri(D-galactosiduronic acid) covalently bound to poly(hydroxyalkyl methacrylate).
PubMed:Lytic enzyme activity in autolysing mycelium of Aspergillus niger.
PubMed:[Characterization of the cleaving mechanism of pectinesterase of Aspergillus niger].
PubMed:[Type of interactions between agarose and Aspergillus niger endopolygalacturonases].
PubMed:[Effect of temperature on production of pectic enzymes with Aspergillus niger in fermentators].
PubMed:Active groups of extracellular endo-D-galacturonanase of aspergillus niger derived from pH effect on kinetic data.
PubMed:[Studies of the chronic toxicity of mold pectinase].
PubMed:Purification and characterisation of polygalacturonases from a commercial Aspergillus niger preparation.
PubMed:The synthesis of pectinases by mutant of Aspergillus niger 36 in beet pulp medium.
PubMed:A study of the inhibitory effects of polyphenols on endo-polygalacturonase from Aspergillus niger.
PubMed:[Research on the degradation mechanism of a purified polygalacturonase from Aspergillus niger].
PubMed:Fungi Associated with Softening of Bisulfite-Brined Cherries.
 
Notes:
Used as a food additive [EAFUS] Used in processing apples, lemons and other fruits and in increasing fruit juice yield. Randomly hydrolyses 1,4-a-D-galactosiduronic linkages in pectate and other galacturonans
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