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methyl isothiazolinone
3(2H)-isothiazolone, 2-methyl-

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CAS Number: 2682-20-4Picture of molecule3D/inchi
ECHA EINECS - REACH Pre-Reg:220-239-6
Nikkaji Web:J34.917H
Beilstein Number:606203
Molecular Weight:115.15505000
Formula:C4 H5 N O S
NMR Predictor:Predict (works with chrome, Edge or firefox)
US / EU / FDA / JECFA / FEMA / FLAVIS / Scholar / Patent Information:
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 FDA/DG SANTE Petitions, Reviews, Notices:
FCN 111 2-Methyl-4-isothiazolin-3-one as a 20 percent solution View - notice
FCN 131 2-Methyl-4-isothiazolin-3-one View - notice
FCN 286 2-Methyl-4-isothiazolin-3-one View - notice
FCN 983 2-methyl-4-isothiazolin-3-one View - notice
FCN 1308 2-Methyl-4-isothiazolin-3-one View - notice
FCN 1649 2-methyl-4-isothiazolin-3-one View - notice
FCN 1733 2-methyl-4-isothiazolin-3-one View - notice
DG SANTE Food Contact Materials:2-methyl-4-isothiazolin-3-one
FDA Mainterm (IAUFC):2682-20-4 ; 2-METHYL-4-ISOTHIAZOLIN-3-ONE
Physical Properties:
Assay: 50.00 to 100.00
Food Chemicals Codex Listed: No
Boiling Point: 182.00 to 183.00 °C. @ 760.00 mm Hg
Vapor Pressure:0.797000 mmHg @ 25.00 °C. (est)
Flash Point: 148.00 °F. TCC ( 64.30 °C. ) (est)
logP (o/w): 0.119 (est)
Soluble in:
 water, 5.367e+005 mg/L @ 25 °C (est)
Organoleptic Properties:
Odor and/or flavor descriptions from others (if found).
Cosmetic Information:
CosIng:cosmetic data
Cosmetic Uses: preservatives
BOC Sciences
For experimental / research use only.
2-Methyl-4-Isothiazoline-3-one 98%
Connect Chemicals
Dow Chemical
Kingyoung Bio Technical
Nanjing Jiancheng Chemical
Odor: characteristic
Use: Methylisothiazolinone is widely used as antiseptic for its bactericidal effect. It can constrain the bacterium, yeast, mould, gram-positive bacterium and gram-negative bacterium. It can be applied in cream, dew liquid, shampoo, hair conditioner, eye cosmetics, liquid cosmetics and other cosmetics.
Santa Cruz Biotechnology
For experimental / research use only.
2-Methyl-4-isothiazolin-3-one ≥98.0%
Sigma-Aldrich: Aldrich
For experimental / research use only.
2-Methyl-4-isothiazolin-3-one 95%
Universal Preserv-A-Chem Inc.
Safety Information:
Preferred SDS: View
Hazards identification
Classification of the substance or mixture
GHS Classification in accordance with 29 CFR 1910 (OSHA HCS)
None found.
GHS Label elements, including precautionary statements
Hazard statement(s)
None found.
Precautionary statement(s)
None found.
Oral/Parenteral Toxicity:
Not determined
Dermal Toxicity:
Not determined
Inhalation Toxicity:
Not determined
Safety in Use Information:
Recommendation for methyl isothiazolinone usage levels up to:
 not for fragrance use.
Recommendation for methyl isothiazolinone flavor usage levels up to:
 not for flavor use.
Safety References:
European Food Safety Authority (EFSA) reference(s):

Opinion of the Scientific Panel on food additives, flavourings, processing aids and materials in contact with food (AFC) related to a 16th list of substances for food contact materials
View page or View pdf

EPI System: View
AIDS Citations:Search
Cancer Citations:Search
Toxicology Citations:Search
EPA Substance Registry Services (TSCA):2682-20-4
EPA ACToR:Toxicology Data
EPA Substance Registry Services (SRS):Registry
Laboratory Chemical Safety Summary :39800
National Institute of Allergy and Infectious Diseases:Data
WGK Germany:3
RTECS:NX8157080 for cas# 2682-20-4
NIST Chemistry WebBook:Search Inchi
Canada Domestic Sub. List:2682-20-4
Pubchem (cid):39800
Pubchem (sid):135019295
Other Information:
(IUPAC):Atomic Weights of the Elements 2011 (pdf)
Videos:The Periodic Table of Videos
tgsc:Atomic Weights use for this web site
(IUPAC):Periodic Table of the Elements
FDA Indirect Additives used in Food Contact Substances:View
HMDB (The Human Metabolome Database):Search
Potential Blenders and core components note
None Found
Potential Uses:
Occurrence (nature, food, other):note
 not found in nature
2-methyl isothiazol-3-one
 skycide 100
isothiazolin-3-one, 2-methyl-
3(2H)-isothiazolone, 2-methyl-


PubMed:Analysis of isothiazolinone preservatives in polyvinyl alcohol cooling towels used in Japan.
PubMed:Methylisothiazolinone in baby wipes: a rising star among causes of contact dermatitis.
PubMed:Six children with allergic contact dermatitis to methylisothiazolinone in wet wipes (baby wipes).
PubMed:Efficacy of metal ions and isothiazolones in inhibiting Enterobacter cloacae BF-17 biofilm formation.
PubMed:Three-phase hollow-fiber liquid-phase microextraction combined with HPLC-UV for the determination of isothiazolinone biocides in adhesives used for food packaging materials.
PubMed:The role of metabotropic glutamate receptor 5 on the stromal cell-derived factor-1/CXCR4 system in oral cancer.
PubMed:Allergic contact dermatitis caused by methylisothiazolinone from different sources, including 'mislabelled' household wet wipes.
PubMed:Methylisothiazolinone contact allergy--growing epidemic.
PubMed:Recommendation to include methylisothiazolinone in the European baseline patch test series--on behalf of the European Society of Contact Dermatitis and the European Environmental and Contact Dermatitis Research Group.
PubMed:Methylisothiazolinone is categorised as a strong sensitiser in the Murine Local Lymph Node Assay.
PubMed:Effects of a 28-day oral exposure to a 5-chloro-2-methyl-4-isothiazolin-3-one and 2-methyl-4-isothiazolin-3-one biocide formulation in Sprague-Dawley rats.
PubMed:Development of allosteric modulators of GPCRs for treatment of CNS disorders.
PubMed:Ubiquitous preservative blamed for rash of skin allergies.
PubMed:Methylisothiazolinone in paint forces a patient out of her apartment.
PubMed:Is 500 ppm a better concentration than 200 ppm for diagnosing contact allergy to methylisothiazolinone?
PubMed:Risk factors associated with methylisothiazolinone contact sensitization.
PubMed:Chronic treatment with MPEP, an mGlu5 receptor antagonist, normalizes basal ganglia glutamate neurotransmission in L-DOPA-treated parkinsonian monkeys.
PubMed:Allergic contact dermatitis to methylisothiazolinone: exposure from baby wipes causing hand dermatitis.
PubMed:Whilst Rome burns: the epidemic of contact allergy to methylisothiazolinone.
PubMed:Methylchloroisothiazolinone and methylisothiazolinone contact allergy: a new 'epidemic'.
PubMed:Airborne exposure to methylchloroisothiazolinone and methylisothiazolinone from a toilet cleaner.
PubMed:Airborne and systemic dermatitis, mimicking atopic dermatitis, caused by methylisothiazolinone in a young child.
PubMed:Methylchloroisothiazolinone and methylisothiazolinone allergic contact dermatitis and the effect of patch test concentration.
PubMed:Airborne exposure to preservative methylisothiazolinone causes severe allergic reactions.
PubMed:Follow-up of the monitored levels of preservative sensitivity in Europe: overview of the years 2001-2008.
PubMed:Recent increase in allergic reactions to methylchloroisothiazolinone/methylisothiazolinone: is methylisothiazolinone the culprit?
PubMed:Total synthesis of epothilones using functionalised allylstannanes for remote stereocontrol.
PubMed:Synthesis and characterization in monkey of [11C]SP203 as a radioligand for imaging brain metabotropic glutamate 5 receptors.
PubMed:Sensitization to methylisothiazolinone in a group of methylchloroisothiazolinone/methylisothiazolinone allergic patients.
PubMed:Contact dermatitis due to methylchloroisothiazolinone/methylisothiazolinone (Kathon CG) as a contaminant in the manufacturing process of a cream.
PubMed:Allergic contact dermatitis caused by methylisothiazolinone from a 'waist reduction belt'.
PubMed:Presumed primary contact sensitization to methylisothiazolinone from paint: a chemical that became airborne.
PubMed:Methylchloroisothiazolinone/methylisothiazolinone contact sensitization: diverging trends in subgroups of IVDK patients in a period of 19 years.
PubMed:Occupational contact dermatitis in painters: an analysis of patch test data from the Danish Contact Dermatitis Group.
PubMed:Abnormal visual processing and increased seizure susceptibility result from developmental exposure to the biocide methylisothiazolinone.
PubMed:Contact allergy to methylchoroisothiazolinone/methylisothiazolinone (MCI/MI): findings from a contact dermatitis unit.
PubMed:[Determination of 3 isothiazolinone preservatives in toys using liquid chromatography-tandem mass spectrometry].
PubMed:Two cases of airborne allergic contact dermatitis caused by methylisothiazolinone in paint.
PubMed:Methylisothiazolinone contact allergy: a review.
PubMed:Methylisothiazolinone contact allergy and dose-response relationships.
PubMed:Contact allergy to methylisothiazolinone in a deodorant.
PubMed:Effects of 5-chloro-2-methyl-4-isothiazolin-3-one and other candidate biodiesel biocides on rat alveolar macrophages and NR8383 cells.
PubMed:Contact sensitization to methylisothiazolinone in Finland--a multicentre study.
PubMed:Moist toilet paper: allergy to the nonhalogenated derivative methylisothiazolinone preservative alone.
PubMed:Final report of the safety assessment of methylisothiazolinone.
PubMed:Methylisothiazolinone, an emerging allergen in cosmetics?
PubMed:The hazards of moist toilet paper: allergy to the preservative methylchloroisothiazolinone/methylisothiazolinone.
PubMed:Delayed hypersensitivity to methylchloroisothiazolinone/methylisothiazolinone not detected by the baseline series of the Spanish group.
PubMed:Concomitant contact allergy to methylchloroisothiazolinone/methylisothiazolinone and formaldehyde-releasing preservatives.
PubMed:Mechanistic assessment of peptide reactivity assay to predict skin allergens with Kathon CG isothiazolinones.
PubMed:Identifying the optimal patch test concentration for methylchloroisothiazolinone and methylisothiazolinone.
PubMed:Expression of surface markers on the human monocytic leukaemia cell line, THP-1, as indicators for the sensitizing potential of chemicals.
PubMed:[Allergic contact dermatitis to cosmetics].
PubMed:Role of intracellular calcium and S-glutathionylation in cell death induced by a mixture of isothiazolinones in HL60 cells.
PubMed:Quantitative relationships between patch test reactivity and use test reactivity: an overview.
PubMed:Alkalization of wall paint prevents airborne contact dermatitis in patients with sensitization to isothiazolinones.
PubMed:Suitability of macrophage inflammatory protein-1beta production by THP-1 cells in differentiating skin sensitizers from irritant chemicals.
PubMed:Antifungal activities of bronopol and 2-methyl-4-isothiazolin-3-one (MT) against saprolegnia.
PubMed:Differential biocide susceptibility of the multiple genotypes of Mycobacterium immunogenum.
PubMed:Cross-reactivity between methylchloroisothiazolinone/methylisothiazolinone, methylisothiazolinone, and other isothiazolinones in workers at a plant producing binders for paints and glues.
PubMed:Metabotropic glutamate 5 receptor (mGluR5) antagonists decrease nicotine seeking, but do not affect the reinforcement enhancing effects of nicotine.
PubMed:'Dermatologically tested' baby toilet tissues: a cause of allergic contact dermatitis in adults.
PubMed:Synthesis and simple 18F-labeling of 3-fluoro-5-(2-(2-(fluoromethyl)thiazol-4-yl)ethynyl)benzonitrile as a high affinity radioligand for imaging monkey brain metabotropic glutamate subtype-5 receptors with positron emission tomography.
PubMed:Iron dependent degradation of an isothiazolone biocide (5-chloro-2-methyl-4-isothiazolin-3-one).
PubMed:Airborne contact dermatitis to methylchloroisothiazolinone/methylisothiazolinone in a boy.
PubMed:An evaluation of dose/unit area and time as key factors influencing the elicitation capacity of methylchloroisothiazolinone/methylisothiazolinone (MCI/MI) in MCI/MI-allergic patients.
PubMed:Contact dermatitis from methylisothiazolinone in a paint factory.
PubMed:Methylisothiazolinone, a neurotoxic biocide, disrupts the association of SRC family tyrosine kinases with focal adhesion kinase in developing cortical neurons.
PubMed:GSH depletion, protein S-glutathionylation and mitochondrial transmembrane potential hyperpolarization are early events in initiation of cell death induced by a mixture of isothiazolinones in HL60 cells.
PubMed:A sensitive radioimmunoassay of insulin autoantibody: reduction of non-specific binding of [125I]insulin.
PubMed:Occupational contact allergy and dermatitis from methylisothiazolinone after contact with wallcovering glue and after a chemical burn from a biocide.
PubMed:Oxidative stress in mouse skin following application of contact allergenic 5-chloro-2-methyl-4-isothiazolin-3-one and oxazolone.
PubMed:From experiment to theory: molecular orbital parameters to interpret the skin sensitization potential of 5-chloro-2-methylisothiazol-3-one and 2-methylisothiazol-3-one.
PubMed:Separation of preservatives in cosmetic products by micellar electrokinetic chromatography.
PubMed:[Allergy to cosmetics. II. Preservatives].
PubMed:Contact sensitization to 5-chloro-2-methyl-4-isothiazolin-3-one and 2-methyl-4-isothiazolin-3-one in children.
PubMed:Covalent binding of the 13C-labeled skin sensitizers 5-chloro-2-methylisothiazol-3-one (MCI) and 2-methylisothiazol-3-one (MI) to a model peptide and glutathione.
PubMed:Involvement of oxidative stress in apoptosis induced by a mixture of isothiazolinones in normal human keratinocytes.
PubMed:Studies of chemical selectivity of hapten, reactivity, and skin sensitization potency. 3. Synthesis and studies on the reactivity toward model nucleophiles of the 13C-labeled skin sensitizers, 5-chloro-2-methylisothiazol-3-one (MCI) and 2-methylisothiazol-3-one (MI).
PubMed:Coupling of contact sensitizers to thiol groups is a key event for the activation of monocytes and monocyte-derived dendritic cells.
PubMed:In vitro neurotoxicity of methylisothiazolinone, a commonly used industrial and household biocide, proceeds via a zinc and extracellular signal-regulated kinase mitogen-activated protein kinase-dependent pathway.
PubMed:Monitoring levels of preservative sensitivity in Europe. A 10-year overview (1991-2000).
PubMed:In vitro induction of apoptosis vs. necrosis by widely used preservatives: 2-phenoxyethanol, a mixture of isothiazolinones, imidazolidinyl urea and 1,2-pentanediol.
PubMed:Preservation of products with MCI/MI in Switzerland.
PubMed:Rôle of methylchloroisothiazolinone/methylisothiazolinone (Kathon CG) in poikiloderma of Civatte.
PubMed:Occupational allergic contact dermatitis from methylchloroisothiazolinone and methylisothiazolinone (MCI/MI) in a silicone-emulsion lock lubricant.
PubMed:Allergic contact dermatitis in patients with anogenital complaints.
PubMed:Occupational allergic contact dermatitis from both 2-bromo-2-nitropropane-1,3-diol and methylchloroisothiazolinone plus methylisothiazolinone in spin finish.
PubMed:Airborne contact dermatitis from methylchloroisothiazolinone in wall paint. Abolition of symptoms by chemical allergen inactivation.
PubMed:Airborne occupational hypersensitivity to isothiazolinones in a papermaking technician.
PubMed:Occupational induction of hypersensitivity after an accidental exposure to chloromethylisothiazolinone and methylisothiazolinone (CMI/MI) in an industrial worker.
PubMed:An update of the risk assessment for methylchloroisothiazolinone/methylisothiazolinone (MCI/MI) with focus on rinse-off products.
PubMed:Occupational allergic contact dermatitis from a glue, containing isothiazolinones and N-methylol-chloroacetamide, in a carpenter.
PubMed:The utility of patch tests using larger screening series of allergens.
PubMed:Can glutathione-containing emollients inactivate methylchloroisothiazolinone/methylisothiazolinone?
PubMed:An epidemic of isothiazolinone sensitization in a flax spinning mill.
PubMed:Can chemical burns and allergic contact dermatitis from higher concentrations of methylchloroisothiazolinone/methylisothiazolinone be prevented?
PubMed:Occupational dermatoses in a plant producing binders for paints and glues.
PubMed:Airborne contact dermatitis due to methylchloro- and methylisothiazolinone (MCI/MI).
PubMed:Sensitization and exposure to methylisothiazolinones (Kathon) in the pulp and paper industry--a report of two cases.
PubMed:Methylisothiazolinones. Diagnosis and prevention of allergic contact dermatitis.
PubMed:Two case reports of delayed skin burns from methylisothiazolines used in water treatment.
PubMed:Formulation effect on the dermal bioavailability of isothiazolone biocide.
PubMed:Mutagenicity of cosmetic products containing Kathon.
PubMed:An unusual case of sensitization to methylchloro- and methyl-isothiazolinone (MCI/MI).
PubMed:Chloromethylisothiazolone/methylisothiazolone (CMI/MI) use test with a shampoo on patch-test-positive subjects. Results of a multicentre double-blind crossover trial.
PubMed:Evaluation of auricular lymph node cell proliferation in isothiazolone-treated mice.
PubMed:Occupational exposure to isothiazolinones. A study based on a product register.
PubMed:Resistance of Pseudomonas aeruginosa to isothiazolone.
PubMed:Delayed skin burns from MCI/MI biocide used in water treatment.
PubMed:Stimulatory effect of cooling tower biocides on amoebae.
PubMed:Allergic contact dermatitis from multiple sources of MCI/MI biocide and formaldehyde in a printer.
PubMed:Occupational dermatitis from isothiazolinones in the nylon production.
PubMed:Multicentre studies and conflicting prevalence data.
PubMed:Allergic contact dermatitis from MCI/MI biocide in a printer.
PubMed:Contact allergy to isothiazolinone derivatives: unusual clinical presentations.
PubMed:Decreased biocide susceptibility of adherent Legionella pneumophila.
PubMed:Assessment of the relative skin sensitizing potency of 3 biocides using the murine local lymph node assay.
PubMed:Isothiazolinones (MCI/MI): 200 ppm versus 100 ppm in the standard series.
PubMed:Relationship between use test and threshold patch test concentration in patients sensitive to 5-chloro-2-methyl-4-isothiazolin-3-one and 2-methyl-4-isothiazolin-3-one (MCI/MI).
PubMed:Contact sensitization to 5-chloro-2-methyl-4-isothiazolin-3-one and 2-methyl-4-isothiazolin-3-one (MCI/MI). A European multicentre study.
PubMed:Kathon CG and cosmetic products.
PubMed:Effects of biocidal treatments to inhibit the growth of legionellae and other microorganisms in cooling towers.
PubMed:Chemical reactivity of some isothiazolone biocides.
PubMed:Growth inhibitory and biocidal activity of some isothiazolone biocides.
PubMed:A patient bothered by unexpected sources of isothiazolinones.
PubMed:Lymphocyte transformation test for diagnosis of isothiazolinone allergy in man.
PubMed:Kathon CG and cosmetic products.
PubMed:An epidemiological study of contact allergy to 5-chloro-3-methyl isothiazolone/3-methyl isothiazolone in Strasbourg.
PubMed:Contact allergy to dichlorinated methylisothiazolinone.
PubMed:The irritancy and allergenicity of 2-n-octyl-4-isothiazolin-3-one (Skane M-8), with recommendations for patch test concentration.
PubMed:Formaldehyde-induced depression of skin reactivity to 5-chloro-2-methyl-4-isothiazolin-3-one in the guinea pig.
PubMed:Kathon CG: a review.
PubMed:Isothiazolinone preservative as important contact allergen in cosmetics.
PubMed:Contact allergy to a contaminant in Kathon CG in the guinea pig.
PubMed:Mutagenic activity associated with cooling tower waters treated with a biocide containing 5-chloro-2-methyl-4-isothiazolin-3-one.
PubMed:Contact allergy to the active ingredients of Kathon CG.
PubMed:Risk of isothiazolinones.
PubMed:Contact allergy to the active ingredients of Kathon CG in the guinea pig.
PubMed:Allergic contact dermatitis due to a biocide containing 5-chloro-2-methyl-4-isothiazolin-3-one.
PubMed:Absorption and disposition of 14C-labelled Kathon biocide, a mixture of 5-chloro-2-methyl-4-isothiazolin-3-one and 2-methyl-4-isothiazolin-3-one, following intravenous or dermal administration to male Sprague-Dawley rats.
PubMed:Kathon CG: cosmetic allergy and patch test sensitization.
PubMed:[Allergic contact eczema caused by isothiazolin-3-one derivatives].
PubMed:Evaluation of some formaldehyde-release compounds and other biocides in the mouse micronucleus test.
PubMed:Kathon biocide: manifestation of delayed contact dermatitis in guinea pigs is dependent on the concentration for induction and challenge.
PubMed:The genetic toxicology of Kathon biocide, a mixture of 5-chloro-2-methyl-4-isothiazolin-3-one and 2-methyl-4-isothiazolin-3-one.
PubMed:Mutagenic activity of Kathon, an industrial biocide and cosmetics preservative containing 5-chloro-2-methyl-4-isothiazolin-3-one and 2-methyl-4-isothiazolin-3-one.
PubMed:Laboratory studies of disinfectants against Legionella pneumophila.
None found
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