| PubMed:RNA Isolation from Mouse Pancreas: A Ribonuclease-rich Tissue. |
| PubMed:Stability of JC Virus DNA in Cerebrospinal Fluid Specimens Preserved with Guanidine Lysis Buffer for Quantitative PCR Testing. |
| PubMed:A comparative study on the aggregating effects of guanidine thiocyanate, guanidine hydrochloride and urea on lysozyme aggregation. |
| PubMed:Production of RNA for transcriptomic analysis from mouse spinal cord motor neuron cell bodies by laser capture microdissection. |
| PubMed:[High-quality total RNA extraction from medicinal plant Aquilaria sinensis]. |
| PubMed:Giardia duodenalis typing from stools: a comparison of three approaches to extracting DNA, and validation of a probe-based real-time PCR typing assay. |
| PubMed:Effect of ionizing radiation on transcription of colorectal cancer MDR1 gene of HCT-8 cells. |
| PubMed:Impact of milk components on recovery of viral RNA from MS2 bacteriophage. |
| PubMed:Development of a rapid total nucleic acid extraction method for the isolation of hepatitis A virus from fresh produce. |
| PubMed:A conductance study of guanidinium chloride, thiocyanate, sulfate, and carbonate in dilute aqueous solutions: ion-association and carbonate hydrolysis effects. |
| PubMed:Distinct effects of guanidine thiocyanate on the structure of superfolder GFP. |
| PubMed:[Comparison of nucleic acid extraction efficiency using different commercial kits and qPCR. Effect of inhibitors]. |
| PubMed:Water plays an important role in osmolyte-induced hairpin structure change: a molecular dynamics simulation study. |
| PubMed:Methods of sperm DNA extraction for genetic and epigenetic studies. |
| PubMed:Accumulation of ACE inhibitory tripeptides, Val-Pro-Pro and Ile-Pro-Pro, in vascular endothelial cells. |
| PubMed:Evaluation of methods for the isolation of high quality RNA from bovine and cervine hide biopsies. |
| PubMed:The proline rich homeodomain protein PRH/Hhex forms stable oligomers that are highly resistant to denaturation. |
| PubMed:A comparison of two approaches to extracting Cryptosporidium DNA from human stools as measured by a real-time PCR assay. |
| PubMed:Evaluation of pathogenic serovars of Leptospira interrogans in dairy cattle herds of Shahrekord by PCR. |
| PubMed:Characterization of intracellular localization of PrP(Sc) in prion-infected cells using a mAb that recognizes the region consisting of aa 119-127 of mouse PrP. |
| PubMed:Evaluation of DNA extraction methods for Bacillus anthracis spores spiked to food and feed matrices at biosafety level 3 conditions. |
| PubMed:Comparison of RNA extraction methods to augment the sensitivity for the differentiation of vesicular stomatitis virus Indiana1 and New Jersey. |
| PubMed:A DNA extraction procedure that allows mite specimens to be slide mounted: phytoseiid species evaluated as a model. |
| PubMed:Analysis of marine bivalve shellfish from the fish market in Santos city, São Paulo state, Brazil, for Toxoplasma gondii. |
| PubMed:Mutant of Moloney murine leukemia virus reverse transcriptase exhibits higher resistance to common RT-qPCR inhibitors. |
| PubMed:[Real-time PCR in analyzing DNA extraction from Cryptosporidium oocysts]. |
| PubMed:Evaluation of two methods for quantification of hsp70 mRNA from the waterborne pathogen Cryptosporidium parvum by reverse transcription real-time PCR in environmental samples. |
| PubMed:Analysis of 7,8-dihydro-8-oxo-2'-deoxyguanosine in cellular DNA during oxidative stress. |
| PubMed:Correlation between the stability and redox potential of three homologous cytochromes c from two thermophiles and one mesophile. |
| PubMed:New method for separation and determination of denatured caseins by hydrophobic interaction chromatography. |
| PubMed:Quantification of mRNA in single cells and modelling of RT-qPCR induced noise. |
| PubMed:RNA extraction from various recalcitrant plant tissues with a cethyltrimethylammonium bromide-containing buffer followed by an acid guanidium thiocyanate-phenol-chloroform treatment. |
| PubMed:An improved RNA isolation method for filamentous fungus Blakeslea trispora rich in polysaccharides. |
| PubMed:Preparation of RNA from tissues and cells. |
| PubMed:[Comparative assessment of DNA extraction methods for identification of glanders and melioidosis etiological agents by PCR]. |
| PubMed:Low cost in-house PCR for the routine diagnosis of extra-pulmonary tuberculosis. |
| PubMed:Remarkable stability of the proton translocating F1FO-ATP synthase from the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1. |
| PubMed:Using silica particles to isolate total RNA from plant tissues recalcitrant to extraction in guanidine thiocyanate. |
| PubMed:Ctenophore tissue preparation and extraction of RNA. |
| PubMed:Development of a molecular diagnostic test applied to experimental abattoir surveillance on bovine tuberculosis. |
| PubMed:IMS-free DNA extraction for the PCR-based quantification of Cryptosporidium parvum and Giardia lamblia in surface and waste water. |
| PubMed:Optimization of DNA isolation from legume nodules. |
| PubMed:A general purpose method for extracting RNA from Dictyostelium cells. |
| PubMed:Virus inactivation by protein denaturants used in affinity chromatography. |
| PubMed:The single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction: twenty-something years on. |
| PubMed:Development of a viral concentration method for bottled water stored in hydrophobic support. |
| PubMed:Effect of prion decontamination protocols on nickel-titanium rotary surfaces. |
| PubMed:Chemical, thermal and pH-induced equilibrium unfolding studies of Fusarium solani lectin. |
| PubMed:[Retention of nucleic acid integrity in guanidine thiocyanate lysates of whole blood]. |
| PubMed:Duplex PCR-hybridization based detection of pathogenic Leptospira in environmental water samples obtained from endemic areas in northeast region of Thailand. |
| PubMed:SPR-based immunocapture approach to creating an interfacial sensing architecture: Mapping of the MRS18-2 binding site on retinoblastoma protein. |
| PubMed:Silica-guanidinium thiocyanate-based nucleic acid isolation protocol does not improve sensitivity of two commercial tests for detection of Mycobacterium tuberculosis in respiratory samples. |
| PubMed:A simple method for detection of rabies viral sequences in 16-year old archival brain specimens with one-week fixation in formalin. |
| PubMed:A method to determine RNA and DNA oxidation simultaneously by HPLC-ECD: greater RNA than DNA oxidation in rat liver after doxorubicin administration. |
| PubMed:Tips for extracting total RNA from chondrocytes cultured in agarose gel using a silica-based membrane kit. |
| PubMed:Nanometer-scale ion aggregates in aqueous electrolyte solutions: guanidinium sulfate and guanidinium thiocyanate. |
| PubMed:Genomic typing of canine parvovirus circulating in the State of Rio de Janeiro, Brazil from 1995 to 2001 using polymerase chain reaction assay. |
| PubMed:Combined spectroscopic and calorimetric characterisation of rubredoxin reversible thermal transition. |
| PubMed:[Fluorescence microscopic study of the microorganisms treated with chaotropic agents]. |
| PubMed:Safe method for isolation of prion protein and diagnosis of Creutzfeldt-Jakob disease. |
| PubMed:Effect of glycosylation on the catalytic and conformational stability of homologous alpha-amylases. |
| PubMed:Detection of acid-fast bacilli in postlysis debris of clinical specimens improves the reliability of PCR. |
| PubMed:Decontamination of surgical instruments from prion proteins: in vitro studies on the detachment, destabilization and degradation of PrPSc bound to steel surfaces. |
| PubMed:A rapid, reliable method to isolate high quality endothelial RNA from small spatially-defined locations. |
| PubMed:Identification of residual structure within denatured antichymotrypsin: implications for serpin folding and misfolding. |
| PubMed:[Changes in the fine structure of microbial cells induced by chaotropic salts]. |
| PubMed:[Creutzfeldt-Jakob disease and other human transmissible spongiform encephalopathies. Part II]. |
| PubMed:Molecular and biochemical assays of cartilage components. |
| PubMed:Detection of piscine nodaviruses by real-time nucleic acid sequence based amplification (NASBA). |
| PubMed:Distribution of hepatitis C virus genotypes in patients with chronic hepatitis C infection in India. |
| PubMed:Direct isolation of poly(A)+ RNA from 4 M guanidine thiocyanate-lysed cell extracts using locked nucleic acid-oligo(T) capture. |
| PubMed:The efficiency of different salts to screen charge interactions in proteins: a Hofmeister effect? |
| PubMed:In vitro evaluation of the anti-prionic activity of newly synthesized congo red derivatives. |
| PubMed:Folding and association of an extremely stable dimeric protein from Sulfolobus islandicus. |
| PubMed:Comparison of storage methods for reverse-transcriptase PCR amplification of rotavirus RNA from gorilla (Gorilla g. gorilla) fecal samples. |
| PubMed:RNA extraction for quantitative enterovirus RT-PCR: comparison of three methods. |
| PubMed:Determination, by dynamic surface-tension analysis, of the molar mass of proteins denatured in guanidine thiocyanate. |
| PubMed:Nucleic acid purification using microfabricated silicon structures. |
| PubMed:Investigating conformational stability of bovine pancreatic phospholipase A2: a novel concept in evaluating the contribution of the 'native-framework' of disulphides to the global conformational stability of proteins. |
| PubMed:Evaluation of a PCR test for the diagnosis of Tritrichomonas foetus infection in bulls: effects of sample collection method, storage and transport medium on the test. |
| PubMed:Structural stability of human alpha-thrombin studied by disulfide reduction and scrambling. |
| PubMed:Characterization of denatured proteins by hydrophobic interaction chromatography: a preliminary study. |
| PubMed:Immunohistochemical investigations of the prion protein accumulation in human spongiform encephalopathies. Special report II. |
| PubMed:Room-temperature hybridization of target DNA with microarrays in concentrated solutions of guanidine thiocyanate. |
| PubMed:Direct extraction of Sabin poliovirus genomes from human fecal samples using a guanidine thiocyanate extraction method. |
| PubMed:Rapid enumeration of Listeria monocytogenes in milk using competitive PCR. |
| PubMed:Separation and determination of denatured alpha(s1)-, alpha(s2)-, beta- and kappa-caseins by hydrophobic interaction chromatography in cows', ewes' and goats' milk, milk mixtures and cheeses. |
| PubMed:Reliable microsatellite genotyping of the Eurasian badger (Meles meles) using faecal DNA. |
| PubMed:Expression of G protein alpha subunits in the lateral wall of the rat cochlea. |
| PubMed:[Effect of varied sample treatment procedures on detection of hepatitis B virus DNA]. |
| PubMed:High-quality genomic DNA from human whole blood and mononuclear cells. |
| PubMed:Comparison of DNA and RNA extraction methods for mummified tissues. |
| PubMed:Cellular background level of 8-oxo-7,8-dihydro-2'-deoxyguanosine: an isotope based method to evaluate artefactual oxidation of DNA during its extraction and subsequent work-up. |
| PubMed:Protease-sensitive scrapie prion protein in aggregates of heterogeneous sizes. |
| PubMed:New chromatographic method for separation and determination of denatured alphaS1-, alphaS2-, beta- and kappa-caseins by hydrophobic interaction chromatography. |
| PubMed:Water contributes actively to the rapid crossing of a protein unfolding barrier. |
| PubMed:Detection of Mycoplasma pneumoniae in spiked clinical samples by nucleic acid sequence-based amplification. |
| PubMed:Optimization of the workup procedure for the analysis of 8-oxo-7,8-dihydro-2'-deoxyguanosine with electrochemical detection. |
| PubMed:The unfolding pathway of leech carboxypeptidase inhibitor. |
| PubMed:Rapid extraction of DNA from diverse soils by guanidine thiocyanate method. |
| PubMed:The unfolding mechanism and the disulfide structures of denatured lysozyme. |
| PubMed:Inhibition of the polymerase chain reaction by sputum samples from tuberculosis patients after processing using a silica-guanidiniumthiocyanate DNA isolation procedure. |
| PubMed:A multivalent assay to detect glycosaminoglycan, protein, collagen, RNA, and DNA content in milligram samples of cartilage or hydrogel-based repair cartilage. |
| PubMed:Thermodynamics of interactions of urea and guanidinium salts with protein surface: relationship between solute effects on protein processes and changes in water-accessible surface area. |
| PubMed:Probing the equilibrium denaturation of the serpin alpha(1)-antitrypsin with single tryptophan mutants; evidence for structure in the urea unfolded state. |
| PubMed:Separation and determination of denatured caseins by hydrophobic interaction chromatography Part II. Method validation and applications. |
| PubMed:High stability of a ferredoxin from the hyperthermophilic archaeon A. ambivalens: involvement of electrostatic interactions and cofactors. |
| PubMed:An efficient method for extraction of RNA from rice leaves at different ages using benzyl chloride. |
| PubMed:Isolation of total RNA from pollens. |
| PubMed:Ribonuclease Rs from Rhizopus stolonifer: lowering of optimum temperature in the presence of urea. |
| PubMed:Portable system for microbial sample preparation and oligonucleotide microarray analysis. |
| PubMed:The structure of denatured alpha-lactalbumin elucidated by the technique of disulfide scrambling: fractionation of conformational isomers of alpha-lactalbumin. |
| PubMed:Synthesis of a stable and specific surface plasmon resonance biosensor surface employing covalently immobilized peptide nucleic acids. |
| PubMed:Unfolding of multimeric proteins in presence of denaturants. A case study of helianthinin from Helianthus annuus L. |
| PubMed:PrP immunohistochemistry: different protocols, including a procedure for long formalin fixation, and a proposed schematic classification for deposits in sporadic Creutzfeldt-Jakob disease. |
| PubMed:Evaluation of bcl-2 gene expression in papilloma of larynx in children. |
| PubMed:The structure of denatured bovine pancreatic trypsin inhibitor (BPTI). |
| PubMed:Analysis of melanoma cells in peripheral blood by reverse transcription-polymerase chain reaction for tyrosinase and MART-1 after mononuclear cell collection with cell preparation tubes: a comparison with the whole blood guanidinium isothiocyanate RNA isolation method. |
| PubMed:The unfolding pathway and conformational stability of potato carboxypeptidase inhibitor. |
| PubMed:A simplified and reliable HPV testing of archival Papanicolaou-stained cervical smears: application to cervical smears from cancer patients starting with cytologically normal smears. |
| PubMed:Rapid and efficient detection of extra-pulmonary Mycobacterium tuberculosis by PCR analysis. |
| PubMed:A rapid method for PCR detection of bovine materials in animal feedstuffs. |
| PubMed:Critical factors influencing the recovery and integrity of rRNA extracted from environmental samples: use of an optimized protocol to measure depth-related biomass distribution in freshwater sediments. |
| PubMed:Creutzfeldt-Jakob disease: Carnoy's fixative improves the immunohistochemistry of the proteinase K-resistant prion protein. |
| PubMed:Antigen retrieval in prion protein immunohistochemistry. |
| PubMed:Detection of bovine spongiform encephalopathy-specific PrP(Sc) by treatment with heat and guanidine thiocyanate. |
| PubMed:A simple and rapid protocol for preparation of crude drug DNA suitable for PCR. |
| PubMed:[Safety measures for handling laboratory specimen and patients with Creutzfeldt-Jakob disease]. |
| PubMed:Analysis of the extent of unfolding of denatured insulin-like growth factor. |
| PubMed:A PCR based method for the identification of equine influenza virus from clinical samples. |
| PubMed:Improved silica-guanidiniumthiocyanate DNA isolation procedure based on selective binding of bovine alpha-casein to silica particles. |
| PubMed:Isolation of high-molecular-weight genomic DNA from intact biohazardous mammalian tissues. |
| PubMed:Denatured states of tick anticoagulant peptide. Compositional analysis of unfolded scrambled isomers. |
| PubMed:Identification of cystatin as a component of carp chorion. |
| PubMed:A comparison of two RNA isolation methods for double-stranded RNA of infectious bursal disease virus. |
| PubMed:Rapid isolation of both double-stranded RNA and PCR-suitable DNA from the obligate biotrophic phytopathogenic fungus Uncinula necator using a commercially available reagent. |
| PubMed:Cleaning CJD-contaminated instruments. |
| PubMed:A nested-PCR-based schizodeme method for identifying Leishmania kinetoplast minicircle classes directly from clinical samples and its application to the study of the epidemiology of Leishmania tropica in Pakistan. |
| PubMed:A fast and inexpensive method for isolation of high-quality RNA from eimerian oocysts. |
| PubMed:Quantitation of porcine reproductive and respiratory syndrome virus RNA in semen by single-tube reverse transcription-nested polymerase chain reaction. |
| PubMed:UV spectrophotometric analysis of ribonucleic acids. |
| PubMed:Isolation of total RNA from tissues or cell lines. Visualization in gel. |
| PubMed:Isolation of total RNA from bacteria. |
| PubMed:Extraction of RNA from fresh and frozen blood. |
| PubMed:Isolation of plant mitochondrial RNA from green leaves. |
| PubMed:Extraction and purification of RNA from plant tissue enriched in polysaccharides. |
| PubMed:RNA extraction from formalin-fixed and paraffin-embedded tissues. |
| PubMed:Quantification of cytokine mRNA expression by RT PCR in samples of previously frozen blood. |
| PubMed:Rapid detection of Mycobacterium paratuberculosis in clinical samples from ruminants and in spiked environmental samples by modified BACTEC 12B radiometric culture and direct confirmation by IS900 PCR. |
| PubMed:The anti-prion activity of Congo red. Putative mechanism. |
| PubMed:Isolation of full-length RNA from a thermophilic cyanobacterium. |
| PubMed:[A simple method for RNA isolation and purification]. |
| PubMed:Detection of toxin-producing cyanobacteria by use of paramagnetic beads for cell concentration and DNA purification. |
| PubMed:Modified guanidinium thiocyanate method for human sperm DNA isolation. |
| PubMed:Isolation of RNA from plant cell suspension cultures and calli by sonication. |
| PubMed:A conformational transition at the N terminus of the prion protein features in formation of the scrapie isoform. |
| PubMed:Efficient recovery of plasmid DNA from Erwinia herbicola with high nuclease activity. |
| PubMed:Foot-and-mouth disease virus and poliovirus particles contain proteins of the replication complex. |
| PubMed:Detection of BK virus in urine by polymerase chain reaction: a comparison of DNA extraction methods. |
| PubMed:Rapid isolation of double-stranded RNAs from entomopathogenic species of the fungus Paecilomyces using a commercial minicolumn system. |
| PubMed:Different expression patterns of somatostatin receptor subtypes in cultured epithelial cells from human normal prostate and prostate cancer. |
| PubMed:Expression of bovine mitochondrial elongation factor Ts in Escherichia coli and characterization of the heterologous complex formed with prokaryotic elongation factor Tu. |
| PubMed:The detection of Escherichia coli DNA in the ancient remains of Lindow Man using the polymerase chain reaction. |
| PubMed:Detection of Francisella tularensis in ulcers of patients with tularemia by PCR. |
| PubMed:Sensitive enzyme-linked immunosorbent assay for detection of PrP(Sc) in crude tissue extracts from scrapie-affected mice. |
| PubMed:DNAzol: a reagent for the rapid isolation of genomic DNA. |
| PubMed:Decontamination of Creutzfeldt-Jakob disease and other transmissible agents. |
| PubMed:Prion protein immunocytochemistry--UK five centre consensus report. |
| PubMed:Isolating RNA from clinical samples with Catrimox-14 and lithium chloride. |
| PubMed:Amplification of Guthrie card DNA: effect of guanidine thiocyanate on binding of natural whole blood PCR inhibitors. |
| PubMed:Detection of Bordetella pertussis in clinical specimens by PCR and a microtiter plate-based DNA hybridization assay. |
| PubMed:Detection of rRNA from four respiratory pathogens using an automated Q beta replicase assay. |
| PubMed:Morphology and toxicity of Abeta-(1-42) dimer derived from neuritic and vascular amyloid deposits of Alzheimer's disease. |
| PubMed:Modification of the acid guanidinium thiocyanate-phenol-chloroform method for nuclear RNA isolation. |
| PubMed:Isolation of HIV-1 RNA from plasma: evaluation of eight different extraction methods. |
| PubMed:A specific and sensitive PCR assay suitable for large-scale detection of toxigenic Pasteurella multocida in nasal and tonsillar swabs specimens of pigs. |
| PubMed:Preferential interaction of denaturants with rice bran lipase. |
| PubMed:Capture and RT-PCR of hepatitis C virus RNA with safety primers. |
| PubMed:Comparison of four RNA extraction methods for the detection of small round structured viruses in faecal specimens. |
| PubMed:A rapid, highly sensitive method for the detection of Francisella tularensis in clinical samples using the polymerase chain reaction. |
| PubMed:Extracellular matrices in peritendinous connective tissue after surgical injury to the chicken flexor tendon. |
| PubMed:Direct solution hybridization of guanidine thiocyanate-solubilized cells for quantitation of mRNAs in hepatocytes. |
| PubMed:Improved RNase protection assay for quantifying LDL-receptor mRNA; estimation of analytical imprecision and biological variance in peripheral blood mononuclear cells. |
| PubMed:RNA extraction from gastrointestinal tract and pancreas by a modified Chomczynski and Sacchi method. |
| PubMed:A simple procedure for gel electrophoresis and northern blotting of RNA. |
| PubMed:Use of Multiscreen plates for the preparation of bacterial DNA suitable for PCR. |
| PubMed:HCV replication in mononuclear cells stimulates anti-HCV-secreting B cells and reflects nonresponsiveness to interferon-alpha. |
| PubMed:Guanidine hydrochloride-induced denaturation of the colicin E1 channel peptide: unfolding of local segments using genetically substituted tryptophan residues. |
| PubMed:New method for the extraction of viral RNA and DNA from cerebrospinal fluid for use in the polymerase chain reaction assay. |
| PubMed:Reverse transcription PCR to detect enteroviruses in surface water. |
| PubMed:Modified method for RNA isolation from bacteria by guanidinium thiocyanate-cesium chloride centrifugation. |
| PubMed:Recovery of RNA from oral streptococci. |
| PubMed:Detection of Mycobacterium tuberculosis directly from spiked human sputum by Q-beta replicase-amplified assay. |
| PubMed:Liquid-phase hybridization and capture of hepatitis B virus DNA with magnetic beads and fluorescence detection of PCR product. |
| PubMed:The influence of antibody levels in dengue diagnosis by polymerase chain reaction. |
| PubMed:Transport of ebselen in plasma and its transfer to binding sites in the hepatocyte. |
| PubMed:Interaction of guanidine hydrochloride and guanidine thiocyanate with wheat germ lipase. |
| PubMed:Prion protein immunocytochemistry: reliable protocols for the investigation of Creutzfeldt-Jakob disease. |
| PubMed:A lysate ribonuclease protection assay. |
| PubMed:Nicotinic acetylcholine receptor subunits expressed in rat cochlea detected by the polymerase chain reaction. |
| PubMed:Effect of surfactants on the diffusion coefficients of proteins, measured by analytical SPLITT fractionation (ASF) in the diffusion mode. |
| PubMed:An improved T1/A ribonuclease protection assay. |
| PubMed:Dissolve and capture: a strategy for analysing mRNA in blood. |
| PubMed:Quantitative measurement of calretinin and beta-actin mRNA [correction of mRNAIN] in rat brain micropunches without prior isolation of RNA. |
| PubMed:Detection of human rhinovirus RNA in nasal washings by PCR. |
| PubMed:A reagent for the single-step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples. |
| PubMed:Development of a rapid and efficient restriction endonuclease analysis typing system for Clostridium difficile and correlation with other typing systems. |
| PubMed:Simple method for extracting RNA from cultured cells and tissue with guanidine salts. |
| PubMed:Ultra-rapid, simple, sensitive, and economical silica method for extraction of dengue viral RNA from clinical specimens and mosquitoes by reverse transcriptase-polymerase chain reaction. |
| PubMed:Detection of HIV-1 in serum, using reverse transcription and the polymerase chain reaction (RT-PCR). |
| PubMed:Expression of insulin-like growth factor-1 in uremic rats: growth hormone resistance and nutritional intake. |
| PubMed:A simple method for isolation of DNA from blood clots suited for use in PCR. |
| PubMed:Absence of protease-resistant prion protein in dementia characterized by neuronal loss and status spongiosus. |
| PubMed:Mechanism of differential inhibition of lactate dehydrogenase isoenzymes in the BMC LD-1 assay. |
| PubMed:A simplified ribonuclease protection assay. |
| PubMed:A faster ribonuclease protection assay. |
| PubMed:Purification of cloned and genomic DNA by guanidine thiocyanate/isobutyl alcohol fractionation. |
| PubMed:Analysis of muscarinic receptor subtypes in the mouse cochlea by means of the polymerase chain reaction. |
| PubMed:RNA isolation from cartilage using density gradient centrifugation in cesium trifluoroacetate: an RNA preparation technique effective in the presence of high proteoglycan content. |
| PubMed:RNA abundance measured by a lysate RNase protection assay. |
| PubMed:Enzyme activation by denaturants in organic solvent systems with a low water content. |
| PubMed:Studies of a tumor-associated antigen, COX-1, recognized by a monoclonal antibody. |
| PubMed:Ultrastructural localization of scrapie prion proteins in cytoplasmic vesicles of infected cultured cells. |
| PubMed:Consistent activation of prorenin mRNA in renal hypertensive rats. |
| PubMed:Cold denaturation and 2H2O stabilization of a staphylococcal nuclease mutant. |
| PubMed:A method for the analysis of newly synthesized tritiated mRNA. |
| PubMed:Plasmid purification by phenol extraction from guanidinium thiocyanate solution: development of an automated protocol. |
| PubMed:Rapid method for direct extraction of mRNA from seeded soils. |
| PubMed:Brain-specific polyA- transcripts are detected in polyA+ RNA: do complex polyA- brain RNAs really exist? |
| PubMed:A novel non-enzymatic procedure for removing DNA template from RNA transcription mixtures. |
| PubMed:Immunoreactivity of cerebral amyloidosis is enhanced by protein denaturation treatments. |
| PubMed:Purification and photoaffinity labeling of the I-Ak histocompatibility molecule. |
| PubMed:Effects of Hg, CH(3)-Hg, and Temperature on the Expression of Mercury Resistance Genes in Environmental Bacteria. |
| PubMed:Simultaneous isolation of total cellular RNA and DNA from tissue culture cells using phenol and lithium chloride. |
| PubMed:Clinical evaluation of an automated chemical inhibition assay for lactate dehydrogenase isoenzyme 1. |
| PubMed:Alkaline serine proteinase from Thermomonospora fusca YX. Stability to heat and denaturants. |
| PubMed:Applications of magnetic beads with covalently attached oligonucleotides in hybridization: isolation and detection of specific measles virus mRNA from a crude cell lysate. |
| PubMed:Detection of dengue viral RNA in mosquito vectors by mixed phase and solution hybridization. |
| PubMed:The folding of staphylococcal nuclease in the presence of methanol or guanidine thiocyanate. |
| PubMed:Estimation of cellular DNA content in cell lysates suitable for RNA isolation. |
| PubMed:Detection of poly(I):poly(C12U), mismatched double-stranded RNA, by rapid solution hybridization: blood values after intravenous infusion. |
| PubMed:A micromethod for the isolation of total RNA from adipose tissue. |
| PubMed:An improvement of the single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. |
| PubMed:Amplified detection of viral nucleic acid at subattomole levels using Q beta replicase. |
| PubMed:Inducible ornithine decarboxylase expression in brain subject to vasogenic oedema after transient ischaemia: relationship to C-fos gene expression. |
| PubMed:Enzymatic amplification of RNA purified from crude cell lysate by reversible target capture. |
| PubMed:Quantitative assays based on the use of replicatable hybridization probes. |
| PubMed:Nucleic acid hybridization assays employing dA-tailed capture probes. I. Multiple capture methods. |
| PubMed:A noise-free molecular hybridization procedure for measuring RNA in cell lysates. |
| PubMed:A novel replicating agent isolated from the human intestinal tract having characteristics shared with Creutzfeldt-Jakob and related agents. |
| PubMed:Quantitation of HIV-1 RNA in blood cells of ARC and AIDS patients. |
| PubMed:Extraction of peptides from guanidine thiocyanate homogenates used to isolate RNA. |
| PubMed:Novel electrophoretic approach to study of poly(A)+RNA pool heterogeneity in different areas of human postmortem brain. |
| PubMed:Preparation and characterization of biotinylated probes for the beta-interferon receptor. |
| PubMed:Quantitative molecular hybridization with unfractionated, solubilized cells using RNA probes and polyacrylamide gel electrophoresis. |
| PubMed:Isolation of intact high-molecular-weight DNA by using guanidine isothiocyanate. |
| PubMed:Molecular hybridization with RNA probes in concentrated solutions of guanidine thiocyanate. |
| PubMed:An improved method for mRNA isolation and characterization of in vitro translation products by Western blotting. |
| PubMed:Isolation of low-molecular-weight proteins from amyloid plaque fibers in Alzheimer's disease. |
| PubMed:Synthesis of specific brain proteins is influenced by testosterone at mRNA level in the neonatal rat. |
| PubMed:Cell-free synthesis of tumor-type poly(A) polymerase. |
| PubMed:Isolation and characterization of milk protein nuclear RNAs in rat mammary gland. |
| PubMed:Isolation and fractionation of total nucleic acids from tissues and cells. |
| PubMed:[Isolation of a biologically active poly(A)+RNA from tissues enriched with polysaccharides]. |
| PubMed:RNA extraction by the guanidine thiocyanate procedure. |
| PubMed:Isolation, partial characterization and translation of mRNAs for chymosin and pepsin, the two main aspartyl proteinases of bovine stomach. |
| PubMed:DNA binding protein profiles in Alzheimer's disease. |
| PubMed:Sensitive quantitative analysis of disulfide bonds in polypeptides and proteins. |
| PubMed:Construction and characterization of type II collagen complementary deoxyribonucleic acid clones. |
| PubMed:Mechanisms for the spontaneous formation of covalently linked polymers of the terminal membranolytic complement protein (C9). |
| PubMed:Isolation of poly A(+) RNA from Schistosoma mansoni and immunoprecipitation of its in vitro translation products. |
| PubMed:Solubilization, isolation, and immunochemical characterization of the major outer membrane protein from Rhodopseudomonas sphaeroides. |
| PubMed:Estimation of the free energy of stabilization of ribonuclease A, lysozyme, alpha-lactalbumin, and myoglobin. |
| PubMed:Induction of kidney metallothionein and metallothionein messenger RNA by zinc and cadmium. |
| PubMed:Preparation and in vitro translation of mRNA from Fasciola hepatica. |
| PubMed:Sexual agglutination factor from Chlamydomonas eugametos. |
| PubMed:The binding of [3H]oestradiol-receptor complexes to calf uterine chromatin. |
| PubMed:The non-detergent solubilization and isolation of intracytoplasmic membrane polypeptides from Rhodopseudomonas sphaeroides. |
| PubMed:The reactivation of human interferons by guanidine thiocyanate. |
| PubMed:An improved method for the rapid isolation of brain ribonucleic acid. |
| PubMed:Characterization of porins from the outer membrane of Salmonella typhimurium. 1. Chemical analysis. |
| PubMed:Tumor-associated antigens of rat moloney sarcoma cells. I. Cell-surface antigens. |
| PubMed:Two-dimensional polyacylamide gel electrophoresis of envelope proteins of Escherichia coli. |
| PubMed:Thermodynamics of the unfolding of alpha-lactalbumin by guanidine thiocyanate. |
| PubMed:Extraction of hydroxyproline-containing proteins and pectic substances from cell walls of growing and non-growing mung bean hypocotyl segments. |
| PubMed:Denaturation of globular proteins by guanidine thiocyanate. II. Intrinsic viscosities in 4.5 M quanidine thiocyanate solutions. |
| PubMed:Denaturation of globular proteins by guanidine thiocyanate. I. Optical rotation in aqueous guanidine thiocyanate solutions. |
| PubMed:[Elution by guanidine thiocyanate of newly synthesized mammalian cell DNA from methylated albumin kieselguhr columns]. |
| PubMed:The use of guanidine thiocyanate to fractionate DNA-like RNA adsorbed on methylated bovine albumin-kieselguhr columns. |
| PubMed:Partial characterization of protocollagen from embryonic cartilage. |
3D/inchi