apoaequorin

Articles:

PubMed:Mouse ChemR23 is expressed in dendritic cell subsets and macrophages, and mediates an anti-inflammatory activity of chemerin in a lung disease model.

PubMed:Plant volatiles regulate the activities of Ca2+ -permeable channels and promote cytoplasmic calcium transients in Arabidopsis leaf cells.

PubMed:Evidence for calcium-mediated perception of plant symbiotic signals in aequorin-expressing Mesorhizobium loti.

PubMed:Nitric oxide triggers specific and dose-dependent cytosolic calcium transients in Arabidopsis.

PubMed:Reconstitution of blue fluorescent protein from recombinant apoaequorin and synthetic coelenteramide.

PubMed:Critical consideration on the relationship between auxin transport and calcium transients in gravity perception of Arabidopsis seedlings.

PubMed:The intrinsic fluorescence of apo-obelin and apo-aequorin and use of its quenching to characterize coelenterazine binding.

PubMed:Aequorin variants with improved bioluminescence properties.

PubMed:Role of calcium in acclimation of the cyanobacterium Synechococcus elongatus PCC 7942 to nitrogen starvation.

PubMed:An early Ca2+ influx is a prerequisite to thaxtomin A-induced cell death in Arabidopsis thaliana cells.

PubMed:Soluble protein expression in E. coli cells using IgG-binding domain of protein A as a solubilizing partner in the cold induced system.

PubMed:Role of neutrophil proteinase 3 and mast cell chymase in chemerin proteolytic regulation.

PubMed:Purinergic-mediated Ca2+ influx in Dictyostelium discoideum.

PubMed:Desensitization and internalization of the human motilin receptor is independent of the C-terminal tail.

PubMed:Infrared reflection-absorption spectroscopy and polarization-modulated infrared reflection-absorption spectroscopy studies of the aequorin langmuir monolayer.

PubMed:Cloning, expression, purification and characterization of an isotype of clytin, a calcium-binding photoprotein from the luminous hydromedusa Clytia gregarium.

PubMed:Differences in motilin receptor desensitization after stimulation with motilin or motilides are due to alternative receptor trafficking.

PubMed:Cytoplasmic calcium increases in response to changes in the gravity vector in hypocotyls and petioles of Arabidopsis seedlings.

PubMed:Bioluminescence immunoassay for angiotensin II using aequorin as a label.

PubMed:Determination of affinity and activity of ligands at the human neuropeptide Y Y4 receptor by flow cytometry and aequorin luminescence.

PubMed:Aequorin luminescence-based assay for 5-hydroxytryptamine (serotonin) type 3 receptor characterization.

PubMed:Cytosolic Ca2+ regulates protein expression in E. coli through release from inclusion bodies.

PubMed:Surface properties of "jellyfish": Langmuir monolayer and Langmuir-Blodgett film studies of recombinant aequorin.

PubMed:Identification of the functional expression of adenosine A3 receptor in pancreas using transgenic mice expressing jellyfish apoaequorin.

PubMed:Imidazole-assisted catalysis of luminescence reaction in blue fluorescent protein from the photoprotein aequorin.

PubMed:Ca2+ transient induced by extracellular changes in osmotic pressure in Arabidopsis leaves: differential involvement of cell wall-plasma membrane adhesion.

PubMed:Fluorescence- and luminescence-based methods for the determination of affinity and activity of neuropeptide Y2 receptor ligands.

PubMed:Transient expression of apoaequorin in zebrafish embryos: extending the ability to image calcium transients during later stages of development.

PubMed:Mechanisms of nitric-oxide-induced increase of free cytosolic Ca2+ concentration in Nicotiana plumbaginifolia cells.

PubMed:[D-1-Nal4]endomorphin-2 is a potent micro-opioid receptor antagonist in the aequorin luminescence-based calcium assay.

PubMed:Primary evidence for involvement of IP3 in heat-shock signal transduction in Arabidopsis.

PubMed:Blue fluorescent protein from the calcium-sensitive photoprotein aequorin: catalytic properties for the oxidation of coelenterazine as an oxygenase.

PubMed:Copper elicits an increase in cytosolic free calcium in cultured tobacco cells.

PubMed:Proteinaceous and oligosaccharidic elicitors induce different calcium signatures in the nucleus of tobacco cells.

PubMed:Mitochondrial biogenesis in mtDNA-depleted cells involves a Ca2+-dependent pathway and a reduced mitochondrial protein import.

PubMed:Comparison of antagonist activity of spantide family at human neurokinin receptors measured by aequorin luminescence-based functional calcium assay.

PubMed:AtGLR3.4, a glutamate receptor channel-like gene is sensitive to touch and cold.

PubMed:Cell cycle dependence of elicitor-induced signal transduction in tobacco BY-2 cells.

PubMed:Blue fluorescent protein from the calcium-sensitive photoprotein aequorin is a heat resistant enzyme, catalyzing the oxidation of coelenterazine.

PubMed:Evidence of a novel cell signaling role for extracellular adenosine triphosphates and diphosphates in Arabidopsis.

PubMed:Substitution of conserved glycine residue by alanine in natural and synthetic neuropeptide ligands causes partial agonism at the stomoxytachykinin receptor.

PubMed:Analysis of nitric oxide signaling functions in tobacco cells challenged by the elicitor cryptogein.

PubMed:Analysis of the transient increase in cytosolic Ca2+ during the action potential of higher plants with high temporal resolution: requirement of Ca2+ transients for induction of jasmonic acid biosynthesis and PINII gene expression.

PubMed:Identification of putative voltage-dependent Ca2+-permeable channels involved in cryptogein-induced Ca2+ transients and defense responses in tobacco BY-2 cells.

PubMed:Circadian and diurnal calcium oscillations encode photoperiodic information in Arabidopsis.

PubMed:Chromatography of isoforms of recombinant apoaequorin and method for the preparation of aequorin.

PubMed:One-step purification and refolding of recombinant photoprotein aequorin by immobilized metal-ion affinity chromatography.

PubMed:Recombinant aequorin as a reporter for receptor-mediated changes of intracellular Ca2+ -levels in Drosophila S2 cells.

PubMed:Assay for intracellular calcium using a codon-optimized aequorin.

PubMed:Analysis and effects of cytosolic free calcium increases in response to elicitors in Nicotiana plumbaginifolia cells.

PubMed:Signal transduction leading to low-temperature tolerance in Arabidopsis thaliana.

PubMed:Bacterial expression of in vivo-biotinylated aequorin for direct application to bioluminometric hybridization assays.

PubMed:The motilin pharmacophore in CHO cells expressing the human motilin receptor.

PubMed:Phospholipase C is required for glucose-induced calcium influx in budding yeast.

PubMed:Adaptation of aequorin functional assay to high throughput screening.

PubMed:Bioluminescence immunoassay for thyroxine employing genetically engineered mutant aequorins containing unique cysteine residues.

PubMed:The lipopolysaccharides of the phytopathogen Xanthomonas campestris pv. campestris induce an oxidative burst reaction in cell cultures of Nicotiana tabacum.

PubMed:Using epitope-aequorin conjugate recognition in immunoassays for complex proteins.

PubMed:Differential mRNA degradation of two beta-tubulin isoforms correlates with cytosolic Ca2+ changes in glucan-elicited soybean cells.

PubMed:Functional characterization of a human receptor for neuropeptide FF and related peptides.

PubMed:A high-throughput glow-type aequorin assay for measuring receptor-mediated changes in intracellular calcium levels.

PubMed:Receptor-mediated increase in cytoplasmic free calcium required for activation of pathogen defense in parsley.

PubMed:The crystal structure of the photoprotein aequorin at 2.3 A resolution.

PubMed:Use of recombinant aequorin to study calcium homeostasis and monitor calcium transients in response to heat and cold shock in cyanobacteria.

PubMed:Apoaequorin monitors degradation of endoplasmic reticulum (ER) proteins initiated by loss of ER Ca(2+).

PubMed:Signal amplification system for DNA hybridization assays based on in vitro expression of a DNA label encoding apoaequorin.

PubMed:An automated aequorin luminescence-based functional calcium assay for G-protein-coupled receptors.

PubMed:A serpentine receptor-dependent, Gbeta- and Ca(2+) influx-independent pathway regulates mitogen-activated protein kinase ERK2 in Dictyostelium.

PubMed:Slow changes in cytosolic free Ca2+ in Escherichia coli highlight two putative influx mechanisms in response to changes in extracellular calcium.

PubMed:The in situ regeneration and extraction of recombinant aequorin from Escherichia coli cells and the purification of extracted aequorin.

PubMed:Role of calreticulin in regulating intracellular Ca2+ storage and capacitative Ca2+ entry in HeLa cells.

PubMed:Recombinant human G protein-coupled lysophosphatidic acid receptors mediate intracellular calcium mobilization.

PubMed:Salicylic acid induces a cytosolic Ca2+ elevation in yeast.

PubMed:Aequorea victoria bioluminescence moves into an exciting new era.

PubMed:Mastoparan induces an increase in cytosolic calcium ion concentration and subsequent activation of protein kinases in tobacco suspension culture cells.

PubMed:Reduced Ca2+ uptake by mitochondria in pyruvate dehydrogenase-deficient human diploid fibroblasts.

PubMed:Expression of apo-aequorin during embryonic development; how much is needed for calcium imaging?

PubMed:A bioluminescent assay for agonist activity at potentially any G-protein-coupled receptor.

PubMed:The use of Renilla luciferase, Oplophorus luciferase, and apoaequorin as bioluminescent reporter protein in the presence of coelenterazine analogues as substrate.

PubMed:Hypoosmotic Shock Induces Increases in Cytosolic Ca2+ in Tobacco Suspension-Culture Cells.

PubMed:Measurement of changes in sarcoplasmic reticulum [Ca2+] in rat tail artery with targeted apoaequorin delivered by an adenoviral vector.

PubMed:A slow sustained increase in cytosolic Ca2+ levels mediates stalk gene induction by differentiation inducing factor in Dictyostelium.

PubMed:Regeneration and luminescence of aequorin in Chinese hamster ovary cells transformed with cDNA for apoaequorin.

PubMed:Recombinant apoaequorin acting as a pseudo-luciferase reports micromolar changes in the endoplasmic reticulum free Ca2+ of intact cells.

PubMed:Cold-shock regulation of the Arabidopsis TCH genes and the effects of modulating intracellular calcium levels.

PubMed:Cytosolic calcium and oxidative plant stress.

PubMed:The moss, Physcomitrella patens, transformed with apoaequorin cDNA responds to cold shock, mechanical perturbation and pH with transient increases in cytoplasmic calcium.

PubMed:Titration of recombinant aequorin with calcium chloride.

PubMed:Truncation of Vargula luciferase still results in retention of luminescence.

PubMed:Cold calcium signaling in Arabidopsis involves two cellular pools and a change in calcium signature after acclimation.

PubMed:Differences in stability of recombinant apoaequorin within subcellular compartments.

PubMed:Circadian oscillations of cytosolic and chloroplastic free calcium in plants.

PubMed:Patterns of free calcium in multicellular stages of Dictyostelium expressing jellyfish apoaequorin.

PubMed:Cause of spectral variation in the luminescence of semisynthetic aequorins.

PubMed:Sequence of the cDNA encoding the Ca(2+)-activated photoprotein obelin from the hydroid polyp Obelia longissima.

PubMed:Calcium waves and development.

PubMed:Oxidative Signals in Tobacco Increase Cytosolic Calcium.

PubMed:Changes in free calcium in the endoplasmic reticulum of living cells detected using targeted aequorin.

PubMed:Intracellular free calcium level and its response to cAMP stimulation in developing Dictyostelium cells transformed with jellyfish apoaequorin cDNA.

PubMed:The relative rate of aequorin regeneration from apoaequorin and coelenterazine analogues.

PubMed:Mass spectrometric evidence for a disulfide bond in aequorin regeneration.

PubMed:Imaging calcium dynamics in living plants using semi-synthetic recombinant aequorins.

PubMed:Measurement of intracellular calcium using bioluminescent aequorin expressed in human cells.

PubMed:Validity of putative calcium binding loops of photoprotein aequorin.

PubMed:Rapid changes of mitochondrial Ca2+ revealed by specifically targeted recombinant aequorin.

PubMed:Wind-induced plant motion immediately increases cytosolic calcium.

PubMed:Monitoring gene expression in Chinese hamster ovary cells using secreted apoaequorin.

PubMed:Use of recombinant biotinylated aequorin in microtiter and membrane-based assays: purification of recombinant apoaequorin from Escherichia coli.

PubMed:A C-terminal proline is required for bioluminescence of the Ca(2+)-binding photoprotein, aequorin.

PubMed:Transgenic plant aequorin reports the effects of touch and cold-shock and elicitors on cytoplasmic calcium.

PubMed:Monitoring of intracellular calcium in Saccharomyces cerevisiae with an apoaequorin cDNA expression system.

PubMed:High-level expression and purification of apoaequorin.

PubMed:Bioluminescent immunoassay using a monomeric Fab'-photoprotein aequorin conjugate.

PubMed:Galactose-dependent expression of the recombinant Ca2(+)-binding photoprotein aequorin in yeast.

PubMed:Photoprotein aequorin: use as a reporter enzyme in studying gene expression in mammalian cells.

PubMed:Bioluminescent immunoassay using a fusion protein of protein A and the photoprotein aequorin.

PubMed:Expression and secretion of aequorin as a chimeric antibody by means of a mammalian expression vector.

PubMed:The enzymology and molecular biology of the Ca2+-activated photoprotein, aequorin.

PubMed:Binding of murine monoclonal antibodies to the active and inactive configurations of aequorin.

PubMed:Overexpression and purification of the recombinant Ca2+-binding protein, apoaequorin.

PubMed:Sequence comparisons of complementary DNAs encoding aequorin isotypes.

PubMed:Site-specific mutagenesis of the calcium-binding photoprotein aequorin.

PubMed:Cloning and expression of the cDNA coding for aequorin, a bioluminescent calcium-binding protein.

PubMed:Resistivity to denaturation of the apoprotein of aequorin and reconstitution of the luminescent photoprotein from the partially denatured apoprotein.

apoaequorin

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