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amyloglucosidase from aspergillus niger

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CAS Number: 9032-08-0
Other(deleted CASRN):109136-54-1
ECHA EC Number:232-877-2
FDA UNII: Search
Category:convert starches to dextrins and glucose in the preparation of starch syrups, dextrose, fruit juices
US / EU / FDA / JECFA / FEMA / FLAVIS / Scholar / Patent Information:
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 FDA/DG SANTE Petitions, Reviews, Notices:
GRN 372 Glucoamylase enzyme preparation from Trichoderma reesei expressing the glucoamylase gene from T. reesei (glucoamylase enzyme preparation) View - notice PDF
GRN 657 Glucoamylase from Penicillum oxalicum produced in Aspergillus niger View - notice PDF
JECFA Food Additive: Amyloglucosidase from Aspergillus niger, var.
Physical Properties:
Appearance:tan to dark brown liquid to powder (est)
Food Chemicals Codex Listed: No
Shelf Life: 12.00 month(s) or longer if stored properly.
Storage:refrigerate in tightly sealed containers.
Organoleptic Properties:
Odor and/or flavor descriptions from others (if found).
Cosmetic Information:
CosIng:cosmetic data
Cosmetic Uses: skin conditioning
BOC Sciences
For experimental / research use only.
Amylase, gluco- 95%
Safety Information:
European information :
Most important hazard(s):
None - None found.
S 02 - Keep out of the reach of children.
S 24/25 - Avoid contact with skin and eyes.
Hazards identification
Classification of the substance or mixture
GHS Classification in accordance with 29 CFR 1910 (OSHA HCS)
None found.
GHS Label elements, including precautionary statements
Hazard statement(s)
None found.
Precautionary statement(s)
None found.
Oral/Parenteral Toxicity:
Not determined
Dermal Toxicity:
Not determined
Inhalation Toxicity:
Not determined
Safety in Use Information:
convert starches to dextrins and glucose in the preparation of starch syrups, dextrose, fruit juices
Recommendation for amyloglucosidase from aspergillus niger usage levels up to:
 not for fragrance use.
Recommendation for amyloglucosidase from aspergillus niger flavor usage levels up to:
 not for flavor use.
Safety References:
European Food Safety Authority (EFSA) reference(s):

Annual report on the preparatory work for the evaluation of food enzymes Toxicological part
View page or View pdf

Safety of the food enzyme glucoamylase from a genetically modified Aspergillus niger (strain NZYM-BF)
View page or View pdf
AIDS Citations:Search
Cancer Citations:Search
Toxicology Citations:Search
EPA Substance Registry Services (TSCA):9032-08-0
EPA ACToR:Toxicology Data
EPA Substance Registry Services (SRS):Registry
National Institute of Allergy and Infectious Diseases:Data
WGK Germany:3
Pubchem (sid):135295970
Other Information:
HMDB (The Human Metabolome Database):Search
FDA Listing of Food Additive Status:View
VCF-Online:VCF Volatile Compounds in Food
Potential Blenders and core components note
None Found
Potential Uses:
None Found
Occurrence (nature, food, other):note
Search PMC Picture
 aspergillus niger
Search PMC Picture
 glucan 1,4-alpha-glucosidase
 glucoamylase enzyme preparation from trichoderma reesei expressing the glucoamylase gene from t. reesei (glucoamylase enzyme preparation)


PubMed:Effects of temperature and additives on the thermal stability of glucoamylase from Aspergillus niger.
PubMed:Purification and biochemical characterisation of glucoamylase from a newly isolated Aspergillus niger: relation to starch processing.
PubMed:The transcriptomic fingerprint of glucoamylase over-expression in Aspergillus niger.
PubMed:Enhanced production of Aspergillus niger laccase-like multicopper oxidases through mRNA optimization of the glucoamylase expression system.
PubMed:Recombinant hepatitis B surface antigen production in Aspergillus niger: evaluating the strategy of gene fusion to native glucoamylase.
PubMed:Improved enzyme production by bio-pellets of Aspergillus niger: targeted morphology engineering using titanate microparticles.
PubMed:Morphology and structural properties of high-amylose rice starch residues hydrolysed by amyloglucosidase.
PubMed:Comparative study of biochemical properties of glucoamylases from the filamentous fungi Penicillium and Aspergillus.
PubMed:Morphology engineering--osmolality and its effect on Aspergillus niger morphology and productivity.
PubMed:Magnetically recyclable, antimicrobial, and catalytically enhanced polymer-assisted "green" nanosystem-immobilized Aspergillus niger amyloglucosidase.
PubMed:Enhancement of enzymatic activity by magnetic spherical polyelectrolyte brushes: a potential recycling strategy for enzymes.
PubMed:Heterogeneity of Aspergillus niger microcolonies in liquid shaken cultures.
PubMed:Iron oxide filled magnetic carbon nanotube-enzyme conjugates for recycling of amyloglucosidase: toward useful applications in biofuel production process.
PubMed:Structure of the catalytic domain of glucoamylase from Aspergillus niger.
PubMed:Heterogenic expression of genes encoding secreted proteins at the periphery of Aspergillus niger colonies.
PubMed:Morphology engineering of Aspergillus niger for improved enzyme production.
PubMed:Reverse micellar extraction of fungal glucoamylase produced in solid-state fermentation culture.
PubMed:Co-conjugation vis-à-vis individual conjugation of α-amylase and glucoamylase for hydrolysis of starch.
PubMed:Chemoenzymatic synthesis, structural study and biological activity of novel indolizidine and quinolizidine iminocyclitols.
PubMed:(1)H NMR spectroscopy for the in vitro understanding of the glycaemic index.
PubMed:Quantitative iTRAQ secretome analysis of Aspergillus niger reveals novel hydrolytic enzymes.
PubMed:Aspergillus niger genome-wide analysis reveals a large number of novel alpha-glucan acting enzymes with unexpected expression profiles.
PubMed:Hydrolysis of native and heat-treated starches at sub-gelatinization temperature using granular starch hydrolyzing enzyme.
PubMed:Evidence that the glucoamylases and alpha-amylase secreted by Aspergillus niger are proteolytically processed products of a precursor enzyme.
PubMed:Overexpression and characterization of Aspergillus awamori wild-type and mutant glucoamylase secreted by the methylotrophic yeast Pichia pastoris: comparison with wild-type recombinant glucoamylase produced using Saccharomyces cerevisiae and Aspergillus niger as hosts.
PubMed:Sweet block copolymer nanoparticles: preparation and self-assembly of fully oligosaccharide-based amphiphile.
PubMed:Industrial glucoamylase fed-batch benefits from oxygen limitation and high osmolarity.
PubMed:[Creation of a heterologous gene expression system on the basis of Aspergillus awamori recombinant strain].
PubMed:Isolation and sequencing of a new glucoamylase gene from an Aspergillus niger aggregate strain (DSM 823) molecularly classified as Aspergillus tubingensis.
PubMed:Directed evolution of Aspergillus niger glucoamylase to increase thermostability.
PubMed:The effect of pH on glucoamylase production, glycosylation and chemostat evolution of Aspergillus niger.
PubMed:Molecular characterization of a PDI-related gene prpA in Aspergillus niger var. awamori.
PubMed:A study of the protein secretory pathway of Aspergillus niger using a glucoamylase-GFP fusion protein.
PubMed:Glucoamylase overexpression and secretion in Aspergillus niger: analysis of glycosylation.
PubMed:Actions of porcine pancreatic and Bacillus subtilis alpha-amylases and Aspergillus niger glucoamylase on phosphorylated (1--4)-alpha-D-glucan.
PubMed:Both binding sites of the starch-binding domain of Aspergillus niger glucoamylase are essential for inducing a conformational change in amylose.
PubMed:Physicochemical properties of rhizome starch from a traditional Chinese medicinal plant of Anemone altaica.
PubMed:A glucoamylase::GFP gene fusion to study protein secretion by individual hyphae of Aspergillus niger.
PubMed:Glucoamylase starch-binding domain of Aspergillus niger B1: molecular cloning and functional characterization.
PubMed:Bioenergetic consequences of glucoamylase production in carbon-limited chemostat cultures of Aspergillus niger.
PubMed:Stabilizing effect of various polyols on the native and the denatured states of glucoamylase.
PubMed:Efficient production from Aspergillus niger of a heterologous protein and an individual protein domain, heavy isotope-labelled, for structure-function analysis.
PubMed:Endoplasmic reticulum stress leads to the selective transcriptional downregulation of the glucoamylase gene in Aspergillus niger.
PubMed:Small angle X-ray studies reveal that Aspergillus niger glucoamylase has a defined extended conformation and can form dimers in solution.
PubMed:Stability and function of interdomain linker variants of glucoamylase 1 from Aspergillus niger.
PubMed:Starch-binding domain shuffling in Aspergillus niger glucoamylase.
PubMed:[Effect of blue light on conidiation development and glucoamylase enhancement in Aspergillus niger].
PubMed:Kinetics of enhanced ethanol productivity using raw starch hydrolyzing glucoamylase from Aspergillus niger mutant produced in solid state fermentation.
PubMed:Glucoamylase::green fluorescent protein fusions to monitor protein secretion in Aspergillus niger.
PubMed:Growth and product formation in chemostat and recycling cultures by Aspergillus niger N402 and a glucoamylase overproducing transformant, provided with multiple copies of the glaA gene.
PubMed:The activity of barley alpha-amylase on starch granules is enhanced by fusion of a starch binding domain from Aspergillus niger glucoamylase.
PubMed:The Effect of organic nitrogen sources on recombinant glucoamylase production by Aspergillus niger in chemostat culture.
PubMed:Physiological optimization of secreted protein production by Aspergillus niger.
PubMed:Improving heterologous gene expression in Aspergillus niger by introducing multiple copies of protein-binding sequence containing CCAAT to the promoter.
PubMed:Enzymatic pretreatment for preparing starch nanocrystals.
PubMed:Function of conserved tryptophans in the Aspergillus niger glucoamylase 1 starch binding domain.
PubMed:Optimization and stability of glucoamylase production by recombinant strains of Aspergillus niger in chemostat culture.
PubMed:Recombinant glucoamylase production by Aspergillus niger B1 in chemostat and pH auxostat cultures.
PubMed:Secretion of active human mucus proteinase inhibitor by Aspergillus niger after KEX2-like processing of a glucoamylase-inhibitor fusion protein.
PubMed:Effect of oxygen enrichment on morphology, growth, and heterologous protein production in chemostat cultures of Aspergillus niger B1-D.
PubMed:In-depth analysis of the Aspergillus niger glucoamylase (glaA) promoter performance using high-throughput screening and controlled bioreactor cultivation techniques.
PubMed:Physicochemical characterisation of the two active site mutants Trp(52)-->Phe and Asp(55)-->Val of glucoamylase from Aspergillus niger.
PubMed:Steady-state inhibitory kinetic studies on the ligand binding modes of Aspergillus niger glucoamylase.
PubMed:Mutational analysis of the roles in catalysis and substrate recognition of arginines 54 and 305, aspartic acid 309, and tryptophan 317 located at subsites 1 and 2 in glucoamylase from Aspergillus niger.
PubMed:Identification, characterization, and partial purification of glucoamylase from Aspergillus niger (syn A. ficuum) NRRL 3135.
PubMed:Production, purification and characterization of the catalytic domain of glucoamylase from Aspergillus niger.
PubMed:Glucoamylase of Aspergillus niger.
PubMed:Some details of the reaction mechanism of glucoamylase from Aspergillus niger--kinetic and structural studies on Trp52-->Phe and Trp317-->Phe mutants.
PubMed:Thermodynamics of inhibitor binding to mutant forms of glucoamylase from Aspergillus niger determined by isothermal titration calorimetry.
PubMed:Irreversible thermoinactivation of glucoamylase from Aspergillus niger and thermostabilization by chemical modification of carboxyl groups.
PubMed:Production and purification of a granular-starch-binding domain of glucoamylase 1 from Aspergillus niger.
PubMed:Expression in Aspergillus niger of the starch-binding domain of glucoamylase. Comparison with the proteolytically produced starch-binding domain.
PubMed:The effect of multiple copies of the upstream region on expression of the Aspergillus niger glucoamylase-encoding gene.
PubMed:Regulation of the glaA gene of Aspergillus niger.
PubMed:Effects of leader sequences upon the heterologous expression of restrictocin in Aspergillus nidulans and Aspergillus niger.
PubMed:Heterologous gene expression in Aspergillus niger: a glucoamylase-porcine pancreatic prophospholipase A2 fusion protein is secreted and processed to yield mature enzyme.
PubMed:Isolation and characterisation of a calnexin homologue, clxA, from Aspergillus niger.
PubMed:Characterization of humanized antibodies secreted by Aspergillus niger.
PubMed:[Use of catabolic repression in the selection of the glucoamylase-producer Aspergillus niger].
PubMed:Production of GFP and glucoamylase by recombinant Aspergillus niger: effects of fermentation conditions on fungal morphology and protein secretion.
PubMed:Localization of growth and secretion of proteins in Aspergillus niger.
PubMed:Glucoamylase production in batch, chemostat and fed-batch cultivations by an industrial strain of Aspergillus niger.
PubMed:Integration of glucoamylase gene from Aspergillus niger into Saccharomyces cerevisiae genome and its stable expression.
PubMed:Evaluation of molecular and genetic approaches to generate glucoamylase overproducing strains of Aspergillus niger.
PubMed:Identification of carboxylic acid residues in glucoamylase G2 from Aspergillus niger that participate in catalysis and substrate binding.
PubMed:Expression and secretion of glucoamylase of Aspergillus niger in Saccharomyces cerevisiae.
PubMed:Metabolomics as a tool for target identification in strain improvement: the influence of phenotype definition.
PubMed:A new method for screening and isolation of hypersecretion mutants in Aspergillus niger.
PubMed:Heterologous expression of the cytotoxin restriction in Aspergillus nidulans and Aspergillus niger.
PubMed:Characterization of the kexin-like maturase of Aspergillus niger.
PubMed:Allergy to Aspergillus-derived enzymes in the baking industry: identification of beta-xylosidase from Aspergillus niger as a new allergen (Asp n 14).
PubMed:Effect of pH on hen egg white lysozyme production and evolution of a recombinant strain of Aspergillus niger.
PubMed:[Glucoamylase enhancement regulated by blue light in Aspergillus niger].
PubMed:[Overproduction of glucoamylase by recombinant Aspergillus niger harboring multiple copies of glaA].
PubMed:Comparison of the properties of glucoamylases from Rhizopus niveus and Aspergillus niger.
PubMed:Releasing of intracellular glucoamylase from Aspergillus niger.
PubMed:Hyphal differentiation in the exploring mycelium of Aspergillus niger.
PubMed:Increased heterologous protein production in Aspergillus niger fermentation through extracellular proteases inhibition by pelleted growth.
PubMed:Study of the glucoamylase promoter in Aspergillus niger using green fluorescent protein.
PubMed:Selection of amylolytically active Aspergillus niger mutants to 2-deoxy-D-glucose.
PubMed:Growth behaviour and glucoamylase production by Aspergillus niger N402 and a glucoamylase overproducing transformant in recycling culture without a nitrogen source.
PubMed:Determination of the maximum product yield from glucoamylase-producing Aspergillus niger grown in the recycling fermentor.
PubMed:Solution structure of the granular starch binding domain of Aspergillus niger glucoamylase bound to beta-cyclodextrin.
PubMed:Site-directed mutagenesis of the catalytic base glutamic acid 400 in glucoamylase from Aspergillus niger and of tyrosine 48 and glutamine 401, both hydrogen-bonded to the gamma-carboxylate group of glutamic acid 400.
PubMed:A truncated glucoamylase gene fusion for heterologous protein secretion from Aspergillus niger.
PubMed:Thermodynamics of binding of heterobidentate ligands consisting of spacer-connected acarbose and beta-cyclodextrin to the catalytic and starch-binding domains of glucoamylase from Aspergillus niger shows that the catalytic and starch-binding sites are in close proximity in space.
PubMed:Purification and characterization of glucoamylase produced by Aspergillus niger in solid state fermentation.
PubMed:Solution structure of the granular starch binding domain of glucoamylase from Aspergillus niger by nuclear magnetic resonance spectroscopy.
PubMed:Chemoenzymatic synthesis of 6 omega-S-alpha-D-glucopyranosyl-6 omega-thiomaltooligosaccharides: their binding to Aspergillus niger glucoamylase G1 and its starch-binding domain.
PubMed:Thermodynamic effects of disulfide bond on thermal unfolding of the starch-binding domain of Aspergillus niger glucoamylase.
PubMed:Use of a diffusion model for mono- and bicomponent anion-exchange of two isoenzymes of glucoamylase from Aspergillus niger in a fixed bed.
PubMed:Molecular dynamics simulations of the unfolding of the starch binding domain from Aspergillus niger glucoamylase.
PubMed:Steady-state kinetic and calorimetric studies on the binding of Aspergillus niger glucoamylase with gluconolactone, 1-deoxynojirimycin, and beta-cyclodextrin.
PubMed:Thermodynamics of ligand binding to the starch-binding domain of glucoamylase from Aspergillus niger.
PubMed:Roles of the aromatic side chains in the binding of substrates, inhibitors, and cyclomalto-oligosaccharides to the glucoamylase from Aspergillus niger probed by perturbation difference spectroscopy, chemical modification, and mutagenesis.
PubMed:The effect of proteases on the synthesis of glucoamylase by mutants of Aspergillus niger C.
PubMed:Substrate binding mechanism of Glu180-->Gln, Asp176-->Asn, and wild-type glucoamylases from Aspergillus niger.
PubMed:Thermodynamics of inhibitor binding to the catalytic site of glucoamylase from Aspergillus niger determined by displacement titration calorimetry.
PubMed:pH-dependence of the fast step of maltose hydrolysis catalysed by glucoamylase G1 from Aspergillus niger.
PubMed:Glucoamylases G1 and G2 from Aspergillus niger are synthesized from two different but closely related mRNAs.
PubMed:Optimization of glucoamylase production by Aspergillus niger in solid-state fermentation.
PubMed:[Effect of the homogeneity of the culture, age and amount of the inoculation matter on glucoamylase biosynthesis by Aspergillus niger strain B 77].
PubMed:Cloning and function determination of promoter region of glucoamylase gene from Aspergillus niger T21.
PubMed:Purification and properties of two forms of glucoamylase from Aspergillus niger.
PubMed:Hen egg white lysozyme expressed in, and secreted from, Aspergillus niger is correctly processed and folded.
PubMed:Deglycosylation of glucoamylase from Aspergillus niger: effects on structure, activity and stability.
PubMed:Reaction mechanisms of Trp120-->Phe and wild-type glucoamylases from Aspergillus niger. Interactions with maltooligodextrins and acarbose.
PubMed:A simple method of affinity chromatography for the purification of glucoamylase obtained from Aspergillus niger C.
PubMed:The promoter of the glucoamylase-encoding gene of Aspergillus niger functions in Ustilago maydis.
PubMed:Action of glucoamylase from Aspergillus niger on phosphorylated substrate.
PubMed:Promoter-tagged restriction enzyme-mediated insertion (PT-REMI) mutagenesis in Aspergillus niger.
PubMed:Can hTNF-alpha be successfully produced and secreted in filamentous fungus Aspergillus niger?
PubMed:Metabolic flux analysis using stoichiometric models for Aspergillus niger: comparison under glucoamylase-producing and non-producing conditions.
PubMed:Determinants of the fidelity of processing glucoamylase-lysozyme fusions by Aspergillus niger.
PubMed:Glucoamylase overexpression in Aspergillus niger: molecular genetic analysis of strains containing multiple copies of the glaA gene.
PubMed:Improvement in raw-starch-digesting glucoamylase production by electrofusion of Aspergillus niger.
PubMed:On-site production of crude glucoamylase for kitchen waste hydrolysis.
PubMed:Stopped-flow fluorescence and steady-state kinetic studies of ligand-binding reactions of glucoamylase from Aspergillus niger.
PubMed:Expression of human lymphotoxin alpha in Aspergillus niger.
PubMed:Comparative studies on extracellular protease secretion and glucoamylase production by free and immobilized Aspergillus niger cultures.
PubMed:Thermodynamics of reversible and irreversible unfolding and domain interactions of glucoamylase from Aspergillus niger studied by differential scanning and isothermal titration calorimetry.
PubMed:Thermal unfolding of the starch binding domain of Aspergillus niger glucoamylase.
PubMed:1H and 15N assignments and secondary structure of the starch-binding domain of glucoamylase from Aspergillus niger.
PubMed:Synthesis, cloning and sequencing of glucoamylase cDNA from Aspergillus niger mutant T21.
PubMed:Binding of isomaltose and maltose to the glucoamylase from Aspergillus niger, as studied by fluorescence spectrophotometry and steady-state kinetics.
PubMed:Investigations on the production of fungal amylolytic enzymes by calcium alginate gel immobilized Aspergillus niger.
PubMed:Characterization of a glucoamylase G2 from Aspergillus niger.
PubMed:Structural studies on the O-glycosidically linked carbohydrate chains of glucoamylase G1 from Aspergillus niger.
PubMed:Transcriptional and post-transcriptional events affect the production of secreted hen egg white lysozyme by Aspergillus niger.
PubMed:An extracellular beta-galactofuranosidase from Aspergillus niger and its use as a tool for glycoconjugate analysis.
PubMed:Flux Design: In silico design of cell factories based on correlation of pathway fluxes to desired properties.
PubMed:Influence of viscosity of enzymatic reactions studied with glucoamylase of Aspergillus niger.
PubMed:The synergetic effects of two CCAAT boxes in Aspergillus niger glaA gene promoter on activation of PglaA transcription.
PubMed:Side chain reactivities of glucoamylase G2 from Aspergillus niger evaluated by group-specific chemical modifications.
PubMed:[Biosynthesis of glucoamylase and the possibilities of using it for the conversion of starch into sugar. I. The effect of the type and concentration of the carbon source on glucoamylase biosynthesis by Aspergillus niger strain B 77].
PubMed:[The use of a glucoamylase enzyme preparation obtained from Aspergillus niger 16/132, for the hydrolysis of corn starch].
PubMed:Studies of the carbohydrate residues of glycoproteins by natural abundance carbon 13 nuclear magnetic resonance spectroscopy. Glucoamylase from Aspergillus niger.
PubMed:Interaction of radical anion probes with glucoamylase I from Aspergillus niger.
PubMed:Properties of a purified thermostable glucoamylase from Aspergillus niveus.
PubMed:Subsite affinities of Aspergillus niger glucoamylase II determined with p-nitrophenylmaltooligosaccharides.
PubMed:Synthesis of 6'-S-alpha-D-glucopyranosyl-6'-thiomaltose (6(2)-thiopanose) and its effect on the enzymic activity of glucoamylases from Aspergillus niger.
PubMed:[Isolation of a highly purified glucoamylase from Aspergillus niger 16/132].
PubMed:[Glucoamylase enzyme preparation from the culture broth of an Aspergillus niger B77 strain. I. The isolation of the crude enzyme preparation and study of its properties].
PubMed:Identification and elimination by site-directed mutagenesis of thermolabile aspartyl bonds in Aspergillus awamori glucoamylase.
PubMed:The action of an amyloglucosidase of Aspergillus niger on starch and malto-oligosaccharides.
PubMed:A CBM20 low-affinity starch-binding domain from glucan, water dikinase.
PubMed:Unconventional layer-by-layer assembly of graphene multilayer films for enzyme-based glucose and maltose biosensing.
PubMed:Identification and characterization of filamentous fungi isolated from fermentation starters for Hong Qu glutinous rice wine brewing.
PubMed:Ethanol production from starch hydrolyzates using Zymomonas mobilis and glucoamylase entrapped in polyvinylalcohol hydrogel.
PubMed:Kinetically trapped metastable intermediate of a disulfide-deficient mutant of the starch-binding domain of glucoamylase.
PubMed:O-glycosylation in Aspergillus glucoamylase. Conformation and role in binding.
PubMed:Refined structure for the complex of acarbose with glucoamylase from Aspergillus awamori var. X100 to 2.4-A resolution.
PubMed:Expression of Aspergillus hemoglobin domain activities in Aspergillus oryzae grown on solid substrates improves growth rate and enzyme production.
PubMed:The glucoamylase cDNA from Aspergillus oryzae: its cloning, nucleotide sequence, and expression in Saccharomyces cerevisiae.
PubMed:Intracellular and extracellular production of proteins in Aspergillus under the control of expression signals of the highly expressed Aspergillus nidulans gpdA gene.
PubMed:Importance of enzyme purity and activity in the measurement of total dietary fiber and dietary fiber components.
PubMed:Differential scanning calorimetric studies on the domain structure of Aspergillus glucoamylase.
PubMed:Complexation of alkyl glycosides with α-cyclodextrin can have drastically different effects on their conversion by glycoside hydrolases.
PubMed:[Studies on the regulation of glucoamylase gene (glaA) expression in A. niger I. The overall analysis and comparison of the expression of glucoamylase in the overproducing strain A. niger T21 and its original strain A. niger 3.795].
PubMed:Amylase activity of Aspergillus strains--producers of organic acids.
PubMed:Cloning, heterologous expression, and enzymatic characterization of a thermostable glucoamylase from Talaromyces emersonii.
PubMed:The hydrolysis of alpha-D-glucosides by amyloglucosidase from Aspergillus niger.
PubMed:[Research on the regulation of glucoamylase gene(glaA) expression in A. niger. II. Analysis of the function of 5'-regulatory region of A. niger T21 and 3.795 glaA gene].
PubMed:Interaction of transport resistances with biochemical reaction in packed-bed solid-state fermentors: effect of temperature gradients.
PubMed:Functional roles of the invariant aspartic acid 55, tyrosine 306, and aspartic acid 309 in glucoamylase from Aspergillus awamori studied by mutagenesis.
PubMed:Improvement of enzyme production in Aspergillus.
PubMed:Direct monitoring of enzymatic glucan hydrolysis on a 27-MHz quartz-crystal microbalance.
PubMed:Use of Aspergillus overproducing mutants, cured for integrated plasmid, to overproduce heterologous proteins.
PubMed:Production of amyloglucosidase by Aspergillus niger under different cultivation regimens.
PubMed:Functional elements of the promoter region of the Aspergillus oryzae glaA gene encoding glucoamylase.
PubMed:Glucoamylase mutants in the conserved active-site segment Trp170-Tyr175 located at a distance from the site of catalysis.
PubMed:Engineering a bifunctional starch-cellulose cross-bridge protein.
PubMed:Energetic and mechanistic studies of glucoamylase using molecular recognition of maltose OH groups coupled with site-directed mutagenesis.
PubMed:Efficient KEX2-like processing of a glucoamylase-interleukin-6 fusion protein by Aspergillus nidulans and secretion of mature interleukin-6.
PubMed:Purification of glucoamylase by acarbose (BAY g-5421) affinity chromatography.
PubMed:Crosslinking of glucoamylases via carbohydrates hardly affects catalysis but impairs stability.
PubMed:Structure and energetics of the glucoamylase-isomaltose transition-state complex probed by using modeling and deoxygenated substrates coupled with site-directed mutagenesis.
PubMed:Thermosensitive mutants of Aspergillus awamori glucoamylase by random mutagenesis: inactivation kinetics and structural interpretation.
PubMed:Refined crystal structures of glucoamylase from Aspergillus awamori var. X100.
PubMed:Refined structure for the complex of 1-deoxynojirimycin with glucoamylase from Aspergillus awamori var. X100 to 2.4-A resolution.
PubMed:[Construction of recipient strain of expression-secretion system in filamentous fungi].
PubMed:Conversion of cassava starch to biomass, carbohydrates, and acids by Aspergillus niger.
PubMed:Functional roles and subsite locations of Leu177, Trp178 and Asn182 of Aspergillus awamori glucoamylase determined by site-directed mutagenesis.
PubMed:Modulation of biorecognition of glucoamylases with Concanavalin A by glycosylation via recombinant expression.
PubMed:Synthesis of 2-deoxy-glucooligosaccharides through condensation of 2-deoxy-D-glucose by glucoamylase and alpha-glucosidase.
PubMed:Improving the amylolytic activity of Saccharomyces cerevisiae glucoamylase by the addition of a starch binding domain.
PubMed:Analysis of the role of the gene bipA, encoding the major endoplasmic reticulum chaperone protein in the secretion of homologous and heterologous proteins in black Aspergilli.
PubMed:A motif of a microbial starch-binding domain found in human genethonin.
PubMed:Isolation of two laccase genes from the white-rot fungus Pleurotus eryngii and heterologous expression of the pel3 encoded protein.
PubMed:The interaction of 1-fluoro-D-glucopyranosyl fluoride with glucosidases.
PubMed:Expression and secretion of alpha-amylase and glucoamylase in Saccharomyces cerevisiae.
PubMed:Glucoamylase: another fungal enzyme associated with baker's asthma.
PubMed:[Monitoring glucoamylase fermentation with infrared spectroscopy].
PubMed:Catalytic mechanism of glucoamylase probed by mutagenesis in conjunction with hydrolysis of alpha-D-glucopyranosyl fluoride and maltooligosaccharides.
PubMed:Glucoamylase gene fusions alleviate limitations for protein production in Aspergillus awamori at the transcriptional and (post) translational levels.
PubMed:[Effect of the composition of the medium and the conditions of Aspergillus foetidus cultivation on the biosynthesis of glucoamylase].
PubMed:One-step purification of glucoamylase by affinity precipitation with alginate.
PubMed:[Isolation and sequencing of glucoamylase gene from a glucoamylase over producing strain].
PubMed:Reversible immobilization of glucoamylase by ionic adsorption on sepabeads coated with polyethyleneimine.
PubMed:Macroaffinity ligand-facilitated three-phase partitioning for purification of glucoamylase and pullulanase using alginate.
PubMed:Evolution of a recombinant (gucoamylase-producing) strain of Fusarium venenatum A3/5 in chemostat culture.
PubMed:The starch-binding domain from glucoamylase disrupts the structure of starch.
PubMed:Isoelectric focusing in a multicompartment electrolyzer with zwitterionic membranes, exemplified by purification of glucoamylase.
PubMed:Cloning, heterologous expression, and characterization of Thielavia terrestris glucoamylase.
PubMed:Combined use of growth rate correlated and growth rate independent promoters for recombinant glucoamylase production in Fusarium venenatum.
PubMed:[The decomposition of Maillard reaction products by amylolytic enzymes. 1. Reversible inhibition of alpha- and glucoamylase and alpha-glucosidase by oligosaccharide Amidori compounds].
PubMed:Structure, function and protein engineering of starch-degrading enzymes.
PubMed:An immunoaffinity procedure for determination of enzyme activity at elevated temperatures.
PubMed:Asymmetric syntheses of (-)-lentiginosine and an original pyrrolizidinic analogue thereof from a versatile epoxyamine intermediate.
PubMed:Isoglucose production from raw starchy materials based on a two-stage enzymatic system.
PubMed:Glucoamylase: structure/function relationships, and protein engineering.
PubMed:Preparative isoelectric focusing of proteins using binary buffers in a vortex-stabilized, free-flow apparatus.
PubMed:Amylolytic hydrolysis of native starch granules affected by granule surface area.
PubMed:Influence of the carbohydrate moiety on the stability of glycoproteins.
PubMed:An improved colony-PCR method for filamentous fungi for amplification of PCR-fragments of several kilobases.
PubMed:Effect of drought stress on the development of endosperm starch granules and the composition and physicochemical properties of starches from soft and hard wheat.
PubMed:Purification, characterization, and substrate specificity of a glucoamylase with steroidal saponin-rhamnosidase activity from Curvularia lunata.
PubMed:Fungal glucoamylases.
PubMed:Structural and functional properties of alkali-treated high-amylose rice starch.
PubMed:Authenticity and reconstitution of immobilized enzymes: characterization and denaturation/renaturation of glucoamylase II.
PubMed:Glucoamylase structural, functional, and evolutionary relationships.
PubMed:Spectroscopic study of the temperature-dependent conformation of glucoamylase.
PubMed:Glycoenzymes: an unusual type of glycoprotein structure for a glucoamylase.
PubMed:Structural and functional properties of C-type starches.
PubMed:Raw starch digestion.
PubMed:Strategies for improving heterologous protein production from filamentous fungi.
PubMed:Construction of a fusion enzyme system by gene splicing as a new molecular recognition element for a sequence biosensor.
PubMed:[Effect of progressive chemical modification on the activity and thermal stability of soluble and immobilized glucoamylase].
PubMed:Quantification of intracellular metabolic fluxes from fractional enrichment and 13C-13C coupling constraints on the isotopomer distribution in labeled biomass components.
PubMed:Specificity of the binding domain of glucoamylase 1.
PubMed:Influence of baking enzymes on antimicrobial activity of five bacteriocin-like inhibitory substances produced by lactic acid bacteria isolated from Lithuanian sourdoughs.
PubMed:Protein buffering in model systems and in whole human saliva.
PubMed:An automated method for the quasi-continuous analysis of degradation and transfer products during the enzymatic hydrolysis of oligosaccharides.
PubMed:NMR spectroscopy of exchangeable protons of glucoamylase and of complexes with inhibitors in the 9-15-ppm range.
PubMed:Separation and direct detection of raw and gelatinized starch hydrolyzing activities of glucoamylase on isoelectric focusing gels.
PubMed:The thermostability of glucoamylase immobilized in different ways has a certain limit.
PubMed:O-glycosylation and stability. Unfolding of glucoamylase induced by heat and guanidine hydrochloride.
PubMed:Construction of a brewing yeast having glucoamylase activity and its fermentation characteristics.
PubMed:Solution structure of family 21 carbohydrate-binding module from Rhizopus oryzae glucoamylase.
PubMed:An expression system based on the promoter region of the Aspergillus awamori 1,4-beta-endoxylanase A gene.
PubMed:A simplified modification of the AOAC official method for determination of total dietary fiber using newly developed enzymes.
PubMed:Genetic transformation of Aureobasidium pullulans.
PubMed:Characterization of a 52 kDa exoantigen of Penicillium chrysogenum and monoclonal antibodies suitable for its detection.
PubMed:[Enzymatic conversion of polymers. Nature of apparent product inhibition in the course of enzymatic degradation of polymer substrates].
PubMed:Inhibition of beta-fructofuranosidases and alpha-glucosidases by synthetic thio-fructofuranoside.
PubMed:Magnetite-alginate beads for purification of some starch degrading enzymes.
PubMed:Possibility for discriminating between two representative non two-state thermal unfolding models of proteins by DSC.
PubMed:Nucleotide sequence and expression of the glucoamylase-encoding gene (glaA) from Aspergillus oryzae.
PubMed:Interaction of beta-cyclodextrin with the granular starch binding domain of glucoamylase.
PubMed:Colourimetric and fluorometric substrates for measurement of pullulanase activity.
PubMed:Interaction of transport resistances with biochemical reaction in packed bed solid state fermenters: the effect of gaseous concentration gradients.
PubMed:Chemoselective capture of glycans for analysis on gold nanoparticles: carbohydrate oxime tautomers provide functional recognition by proteins.
PubMed:Modelling of glucoamylase thermal inactivation in the presence of starch by artificial neural network.
PubMed:Active site similarities of glucose dehydrogenase, glucose oxidase, and glucoamylase probed by deoxygenated substrates.
PubMed:[Effect of acid alpha-glucosidase from animal tissues on structurally different oligosaccharides].
PubMed:Inhibition of human digestive enzymes by hydrogenated malto-oligosaccharides.
PubMed:Thermal stabilization of glucose oxidase and glucoamylase by physical entrapment.
PubMed:[Substrate thermostabilization of soluble and immobilized glucoamylase].
PubMed:Growth-rate-independent production of recombinant glucoamylase by Fusarium venenatum JeRS 325.
PubMed:Cloning of Corticium rolfsii glucoamylase cDNA and its expression in Saccharomyces cerevisiae.
PubMed:Kinetics of enzymatic hydrolysis of polysaccharides.
PubMed:Synthesis of alkylated deoxynojirimycin and 1,5-dideoxy-1,5-iminoxylitol analogues: polar side-chain modification, sulfonium and selenonium heteroatom variants, conformational analysis, and evaluation as glycosidase inhibitors.
PubMed:Concise asymmetric syntheses of (-)-lentiginosine and of its pyrrolizidinic analogue.
PubMed:Structure of the arabinogalactan from gum tragacanth (Astralagus gummifer).
PubMed:Protein partitioning equilibrium between the aqueous poly(ethylene glycol) and salt phases and the solid protein phase in poly(ethylene glycol)-salt two-phase systems.
PubMed:Isolation of glycosidase-inhibiting hyacinthacines and related alkaloids from Scilla socialis.
PubMed:Transglycosylation of glycosyl residues to cyclic tetrasaccharide by Bacillus stearothermophilus cyclomaltodextrin glucanotransferase using cyclomaltodextrin as the glycosyl donor.
PubMed:Influence of mannan epitopes in glycoproteins--Concanavalin A interaction. Comparison of natural and synthetic glycosylated proteins.
PubMed:Starch biosynthesis: mechanism for the elongation of starch chains.
PubMed:Air-dried enzyme electrodes.
PubMed:Natural-abundance 13C-n.m.r. spectra of some model compounds for uncommon glycopeptide linkages in glycoproteins.
PubMed:Specific empirical free energy function for automated docking of carbohydrates to proteins.
PubMed:Production of Fusarium solani f. sp. pisi cutinase in Fusarium venenatum A3/5.
PubMed:Identification and characterization of glucoamylase from the fungus Thermomyces lanuginosus.
PubMed:Expression of the cefG gene is limiting for cephalosporin biosynthesis in Acremonium chrysogenum.
PubMed:GENFOLD: a genetic algorithm for folding protein structures using NMR restraints.
PubMed:Lentiginosine, a dihydroxyindolizidine alkaloid that inhibits amyloglucosidase.
PubMed:Immobilization of enzymes based on hydrophobic interaction. II. Preparation and properties of an amyloglucosidase adsorbate.
PubMed:Amyloglucosidase enzymatic reactivity inside lipid vesicles.
PubMed:Carbohydrate content and regulation following injection of different glycogenolytic enzymes.
PubMed:Sensitization to wheat flour and enzymes and associated respiratory symptoms in British bakers.
PubMed:Hydrolysis of glycosylpyridinium ions by anomeric-configuration-inverting glycosidases.
PubMed:Gas concentration and temperature gradients in a packed bed solid-state fermentor.
PubMed:No Latex Starch Utilization in Euphorbia esula L.
An enzyme that catalyzes the hydrolysis of terminal 1,4-linked alpha-D-glucose residues successively from non-reducing ends of polysaccharide chains with the release of beta-glucose. It is also able to hydrolyze 1,6-alpha-glucosidic bonds when the next bond in sequence is 1,4. an enzyme that catalyzes the hydrolysis of terminal 1,4-linked alpha-d-glucose residues successively from non-reducing ends of polysaccharide chains with the release of beta-glucose. it is also able to hydrolyze 1,6-alpha-glucosidic bonds when the next bond in sequence is 1,4. Used in the manuf. of glucose and fructose syrups, in brewing low calorie beers, and in increasing fruit juice yields. Hydrolyses terminal 1,4-linked a-D-glucose residues successively from non-reducing ends of polysaccharide chains with release of b-D-glucose; acts on amylose and amylopectin [DFC]
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